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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Isolation of Human Lineage, Fluoroquinolone-Resistant and Extended-beta-Lactamase-Producing Escherichia coli Isolates from Companion Animals in Japan
| Title: | Isolation of Human Lineage, Fluoroquinolone-Resistant and Extended-beta-Lactamase-Producing Escherichia coli Isolates from Companion Animals in Japan |
| Authors: | Sato, Toyotaka Browse this author →KAKEN DB | | Yokota, Shin-ichi Browse this author | | Tachibana, Tooru Browse this author | | Tamai, Satoshi Browse this author | | Maetani, Shigeki Browse this author | | Tamura, Yutaka Browse this author | | Horiuchi, Motohiro Browse this author →KAKEN DB |
| Keywords: | fluoroquinolone-resistance | | ESBL | | Escherichia coli | | dogs | | cats |
| Issue Date: | Dec-2021 |
| Publisher: | MDPI |
| Journal Title: | Antibiotics-Basel |
| Volume: | 10 |
| Issue: | 12 |
| Start Page: | 1463 |
| Publisher DOI: | 10.3390/antibiotics10121463 |
| Abstract: | An increase in human and veterinary fluoroquinolone-resistant Escherichia coli is a global concern. In this study, we isolated fluoroquinolone-resistant E. coli isolates from companion animals and characterized them using molecular epidemiological analysis, multiplex polymerase chain reaction to detect E. coli ST131 and CTX-M type extended-spectrum beta-lactamases (ESBL), and multi-locus sequence typing analysis. Using plain-CHROMagar ECC, 101 E. coli isolates were isolated from 34 rectal swabs of dogs and cats. The prevalence of resistance to fluoroquinolone and cefotaxime was 27.7% and 24.8%, respectively. The prevalence of fluoroquinolone-resistant isolates (89.3%) was higher when CHROMagar ECC with CHROMagar ESBL supplement was used for E. coli isolation. The prevalence of cefotaxime resistance was also higher (76.1%) when 1 mg/L of ciprofloxacin-containing CHROMagar ECC was used for isolation. The cefotaxime-resistant isolates possessed CTX-M type beta-lactamase genes (CTX-M-14, CTX-M-15, or CTX-M-27). Seventy-five percent of fluoroquinolone-resistant isolates were sequence types ST131, ST10, ST1193, ST38, or ST648, which are associated with extensive spread in human clinical settings. In addition, we isolated three common fluoroquinolone-resistant E. coli lineages (ST131 clade C1-M-27, C1-nM27 and ST2380) from dogs and their respective owners. These observations suggest that companion animals can harbor fluoroquinolone-resistant and/or ESBL-producing E. coli, in their rectums, and that transmission of these isolates to their owners can occur. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/84080 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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