Title: | Whole-Genome Sequencing Reveals Recent Transmission of Multidrug-Resistant Mycobacterium tuberculosis CAS1-Kili Strains in Lusaka, Zambia |
Authors: | Chizimu, Joseph Yamweka Browse this author |
Solo, Eddie Samuneti Browse this author |
Bwalya, Precious Browse this author |
Tanomsridachchai, Wimonrat Browse this author |
Chambaro, Herman Browse this author |
Shawa, Misheck Browse this author |
Kapalamula, Thoko Flav Browse this author |
Lungu, Patrick Browse this author |
Fukushima, Yukari Browse this author |
Mukonka, Victor Browse this author |
Thapa, Jeewan Browse this author |
Nakajima, Chie Browse this author →KAKEN DB |
Suzuki, Yasuhiko Browse this author →KAKEN DB |
Keywords: | Mycobacterium tuberculosis |
CAS-Kili |
recent transmission |
multidrug resistance |
whole-genome sequencing |
Issue Date: | Jan-2022 |
Publisher: | MDPI |
Journal Title: | Antibiotics-Basel |
Volume: | 11 |
Issue: | 1 |
Start Page: | 29 |
Publisher DOI: | 10.3390/antibiotics11010029 |
Abstract: | Globally, tuberculosis (TB) is a major cause of death due to antimicrobial resistance. Mycobacterium tuberculosis CAS1-Kili strains that belong to lineage 3 (Central Asian Strain, CAS) were previously implicated in the spread of multidrug-resistant (MDR)-TB in Lusaka, Zambia. Thus, we investigated recent transmission of those strains by whole-genome sequencing (WGS) with Illumina MiSeq platform. Twelve MDR CAS1-Kili isolates clustered by traditional methods (MIRU-VNTR and spoligotyping) were used. A total of 92% (11/12) of isolates belonged to a cluster (<= 12 SNPs) while 50% (6/12) were involved in recent transmission events, as they differed by <= 5 SNPs. All the isolates had KatG Ser315Thr (isoniazid resistance), EmbB Met306 substitutions (ethambutol resistance) and several kinds of rpoB mutations (rifampicin resistance). WGS also revealed compensatory mutations including a novel deletion in embA regulatory region (-35A > del). Several strains shared the same combinations of drug-resistance-associated mutations indicating transmission of MDR strains. Zambian strains belonged to the same clade as Tanzanian, Malawian and European strains, although most of those were pan-drug-susceptible. Hence, complimentary use of WGS to traditional epidemiological methods provides an in-depth insight on transmission and drug resistance patterns which can guide targeted control measures to stop the spread of MDR-TB. |
Type: | article |
URI: | http://hdl.handle.net/2115/84184 |
Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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