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Whole-Genome Sequencing Reveals Recent Transmission of Multidrug-Resistant Mycobacterium tuberculosis CAS1-Kili Strains in Lusaka, Zambia

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Title: Whole-Genome Sequencing Reveals Recent Transmission of Multidrug-Resistant Mycobacterium tuberculosis CAS1-Kili Strains in Lusaka, Zambia
Authors: Chizimu, Joseph Yamweka Browse this author
Solo, Eddie Samuneti Browse this author
Bwalya, Precious Browse this author
Tanomsridachchai, Wimonrat Browse this author
Chambaro, Herman Browse this author
Shawa, Misheck Browse this author
Kapalamula, Thoko Flav Browse this author
Lungu, Patrick Browse this author
Fukushima, Yukari Browse this author
Mukonka, Victor Browse this author
Thapa, Jeewan Browse this author
Nakajima, Chie Browse this author →KAKEN DB
Suzuki, Yasuhiko Browse this author →KAKEN DB
Keywords: Mycobacterium tuberculosis
recent transmission
multidrug resistance
whole-genome sequencing
Issue Date: Jan-2022
Publisher: MDPI
Journal Title: Antibiotics-Basel
Volume: 11
Issue: 1
Start Page: 29
Publisher DOI: 10.3390/antibiotics11010029
Abstract: Globally, tuberculosis (TB) is a major cause of death due to antimicrobial resistance. Mycobacterium tuberculosis CAS1-Kili strains that belong to lineage 3 (Central Asian Strain, CAS) were previously implicated in the spread of multidrug-resistant (MDR)-TB in Lusaka, Zambia. Thus, we investigated recent transmission of those strains by whole-genome sequencing (WGS) with Illumina MiSeq platform. Twelve MDR CAS1-Kili isolates clustered by traditional methods (MIRU-VNTR and spoligotyping) were used. A total of 92% (11/12) of isolates belonged to a cluster (<= 12 SNPs) while 50% (6/12) were involved in recent transmission events, as they differed by <= 5 SNPs. All the isolates had KatG Ser315Thr (isoniazid resistance), EmbB Met306 substitutions (ethambutol resistance) and several kinds of rpoB mutations (rifampicin resistance). WGS also revealed compensatory mutations including a novel deletion in embA regulatory region (-35A > del). Several strains shared the same combinations of drug-resistance-associated mutations indicating transmission of MDR strains. Zambian strains belonged to the same clade as Tanzanian, Malawian and European strains, although most of those were pan-drug-susceptible. Hence, complimentary use of WGS to traditional epidemiological methods provides an in-depth insight on transmission and drug resistance patterns which can guide targeted control measures to stop the spread of MDR-TB.
Type: article
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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