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Binocular stereo-microscopy for deforming intact amoeba
Title: | Binocular stereo-microscopy for deforming intact amoeba |
Authors: | Matsumoto, Kenji Browse this author →KAKEN DB | Nishigami, Yukinori Browse this author →KAKEN DB | Nakagaki, Toshiyuki Browse this author →KAKEN DB |
Issue Date: | 17-Jan-2022 |
Publisher: | Optical Society of America |
Journal Title: | Optics express |
Volume: | 30 |
Issue: | 2 |
Start Page: | 2424 |
End Page: | 2437 |
Publisher DOI: | 10.1364/OE.439825 |
Abstract: | A powerful and convenient method for measuring three-dimensional (3D) deformation of moving amoeboid cells will assist the progress of environmental and cytological studies as protists amoebae play a role in the fundamental environmental ecosystem. Here we develop an inexpensive and useful method for measuring 3D deformation of single protists amoeba through binocular microscopy and a newly proposed algorithm of stereo-scopy. From the movies taken from the left and right optical tubes of the binocular microscope, we detect the 3D positions of many intrinsic intracellular vesicles and reconstruct cellular surfaces of amoeboid cells in 3D space. Some observations of sampled behaviors are shown in a single-celled organism of Amoeba proteus. The resultant surface time series is then analyzed to obtain surface velocity, curvature and volume increasing rates of pseudo-pods for characterizing the movements of amoeboid cells. The limitations and errors of this method are also discussed. (C) 2022 Optical Society of America under the terms of the OSA Open Access Publishing Agreement |
Type: | article |
URI: | http://hdl.handle.net/2115/84186 |
Appears in Collections: | 電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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