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Binocular stereo-microscopy for deforming intact amoeba

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Title: Binocular stereo-microscopy for deforming intact amoeba
Authors: Matsumoto, Kenji Browse this author →KAKEN DB
Nishigami, Yukinori Browse this author →KAKEN DB
Nakagaki, Toshiyuki Browse this author →KAKEN DB
Issue Date: 17-Jan-2022
Publisher: Optical Society of America
Journal Title: Optics express
Volume: 30
Issue: 2
Start Page: 2424
End Page: 2437
Publisher DOI: 10.1364/OE.439825
Abstract: A powerful and convenient method for measuring three-dimensional (3D) deformation of moving amoeboid cells will assist the progress of environmental and cytological studies as protists amoebae play a role in the fundamental environmental ecosystem. Here we develop an inexpensive and useful method for measuring 3D deformation of single protists amoeba through binocular microscopy and a newly proposed algorithm of stereo-scopy. From the movies taken from the left and right optical tubes of the binocular microscope, we detect the 3D positions of many intrinsic intracellular vesicles and reconstruct cellular surfaces of amoeboid cells in 3D space. Some observations of sampled behaviors are shown in a single-celled organism of Amoeba proteus. The resultant surface time series is then analyzed to obtain surface velocity, curvature and volume increasing rates of pseudo-pods for characterizing the movements of amoeboid cells. The limitations and errors of this method are also discussed. (C) 2022 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Type: article
Appears in Collections:電子科学研究所 (Research Institute for Electronic Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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