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Intermittent PTH Administration Increases Bone-Specific Blood Vessels and Surrounding Stromal Cells in Murine Long Bones

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Title: Intermittent PTH Administration Increases Bone-Specific Blood Vessels and Surrounding Stromal Cells in Murine Long Bones
Authors: Zhao, Shen Browse this author
Hasegawa, Tomoka Browse this author →KAKEN DB
Hongo, Hiromi Browse this author →KAKEN DB
Yamamoto, Tomomaya Browse this author
Abe, Miki Browse this author
Yoshida, Taiji Browse this author
Haraguchi, Mai Browse this author
de Freitas, Paulo Henrique Luiz Browse this author
Li, Minqi Browse this author →KAKEN DB
Tei, Kanchu Browse this author →KAKEN DB
Amizuka, Norio Browse this author →KAKEN DB
Keywords: Blood vessel
Parathyroid hormone (PTH)
Vascular smooth muscle cell
Issue Date: Mar-2021
Publisher: Springer
Journal Title: Calcified tissue international
Volume: 108
Issue: 3
Start Page: 391
End Page: 406
Publisher DOI: 10.1007/s00223-020-00776-2
PMID: 33170307
Abstract: To verify whether PTH acts on bone-specific blood vessels and on cells surrounding these blood vessels, 6-week-old male mice were subjected to vehicle (control group) or hPTH [1-34] (20 mu g/kg/day, PTH group) injections for 2 weeks. Femoral metaphyses were used for histochemical and immunohistochemical studies. In control metaphyses, endomucin-positive blood vessels were abundant, but alpha SMA-reactive blood vessels were scarce. In the PTH-administered mice, the lumen of endomucin-positive blood vessels was markedly enlarged. Moreover, many alpha SMA-positive cells were evident near the blood vessels, and seemed to derive from those vessels. These alpha SMA-positive cells neighboring the blood vessels showed features of mesenchymal stromal cells, such as immunopositivity for c-kit and tissue nonspecific alkaline phosphatase (TNALP). Thus, PTH administration increased the population of perivascular/stromal cells positive for alpha SMA and c-kit, which were likely committed to the osteoblastic lineage. To understand the cellular events that led to increased numbers and size of bone-specific blood vessels, we performed immunohistochemical studies for PTH/PTHrP receptor and VEGF. After PTH administration, PTH/PTHrP receptor, VEGF and its receptor flk-1 were consistently identified in both osteoblasts and blood vessels (endothelial cells and surrounding perivascular cells). Our findings suggest that exogenous PTH increases the number and size of bone-specific blood vessels while fostering perivascular/stromal cells positive for alpha SMA/TNALP/c-kit.
Rights: This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at:
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷川 智香

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