|
Hokkaido University Collection of Scholarly and Academic Papers >
Center for Environmental and Health Sciences >
Peer-reviewed Journal Articles, etc >
Short-term temporal variability of urinary biomarkers of organophosphate flame retardants and plasticizers
| Title: | Short-term temporal variability of urinary biomarkers of organophosphate flame retardants and plasticizers |
| Authors: | Bastiaensen, Michiel Browse this author | | Gys, Celine Browse this author | | Malarvannan, Govindan Browse this author | | Fotache, Mihai Browse this author | | Bombeke, Jasper Browse this author | | Ait Bamai, Yu Browse this author →KAKEN DB | | Araki, Atsuko Browse this author →KAKEN DB | | Covaci, Adrian Browse this author |
| Keywords: | Organophoshate flame retardants | | Variability | | Urine | | ICC | | Exposure assessment |
| Issue Date: | Jan-2021 |
| Publisher: | Elsevier |
| Journal Title: | Environment International |
| Volume: | 146 |
| Start Page: | 106147 |
| Publisher DOI: | 10.1016/j.envint.2020.106147 |
| Abstract: | Background
Exposure to organophosphate flame retardants and plasticizers (PFRs) is commonly estimated by measuring biomarker concentrations in spot urine samples. However, their concentrations in urine can vary greatly over time due to short biological half-lives and variable exposure, potentially leading to exposure misclassification. In this study, we examined the within- and between-individual and within- and between-day variability of PFR metabolites in spot and 24-hour pooled urine samples during five consecutive days.
Methods
We collected all spot urine samples from 10 healthy adults for 5 days. On one additional day, we collected 24-hour pooled urine samples. Samples were analyzed by solid-phase extraction coupled to high-performance liquid chromatography tandem mass spectrometry. We calculated intraclass correlation coefficients (ICCs) to assess the reproducibility of metabolite concentrations in morning void and spot samples.
Results
Fair-to-good reproducibility was observed for serial measurements of bis(1,3-dichloro-2-propyl) phosphate (BDCIPP), 1-hydroxy-2-propyl bis(1-chloro-2-propyl) phosphate (BCIPHIPP), 2-hydroxyethyl bis(2-butoxyethyl) phosphate (BBOEHEP) and 2-ethyl-5-hydroxyhexyl diphenyl phosphate (5-HO-EHDPHP) (ICC: 0.396 – 0.599), whereas concentrations of diphenyl phosphate (DPHP) and 2-ethylhexyl phenyl phosphate (EHPHP) were more variable in time (ICC: 0.303 and 0.234). Reproducibility improved significantly when only morning void samples were considered and when concentrations were adjusted for urinary dilution. Collecting 24-hour pooled urine could be a reliable alternative for PFR biomarkers with poor short-term temporal variability.
Conclusions
The between-day variability was minor compared to variability observed within the same day, which suggests that collecting multiple samples could reduce exposure missclassification. Differences in the observed between- and within-individual variance were compound specific and related to both the nature of the exposure (e.g., diet vs other exposure routes, multiple sources) and the individual toxicokinetic properties of the investigated PFRs. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/84307 |
| Appears in Collections: | 環境健康科学研究教育センター (Center for Environmental and Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|
