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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Simultaneous steroids measurement in dogs with hyperadrenocorticism using a column-switching liquid chromatography-tandem mass spectrometry method
| Title: | Simultaneous steroids measurement in dogs with hyperadrenocorticism using a column-switching liquid chromatography-tandem mass spectrometry method |
| Authors: | Sasaki, Noboru Browse this author →KAKEN DB | | Oo, Thandar Browse this author | | Yasuda, Yoshikazu Browse this author | | Ichise, Takahiro Browse this author | | Nagata, Noriyuki Browse this author | | Yokoyama, Nozomu Browse this author | | Sasaoka, Kazuyoshi Browse this author →KAKEN DB | | Morishita, Keitaro Browse this author →KAKEN DB | | Nakayama, Shouta Mm Browse this author | | Ishizuka, Mayumi Browse this author →KAKEN DB | | Nakamura, Kensuke Browse this author →KAKEN DB | | Takiguchi, Mitsuyoshi Browse this author →KAKEN DB | | Ikenaka, Yoshinori Browse this author →KAKEN DB |
| Keywords: | column | | switching | | dog | | hyperadrenocorticism | | liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) | | steroid profile |
| Issue Date: | 31-Oct-2021 |
| Publisher: | 公益社団法人 日本獣医学会 (The Japanese Society of Veterinary Science) |
| Journal Title: | Journal of veterinary medical science |
| Volume: | 83 |
| Issue: | 11 |
| Start Page: | 1634 |
| End Page: | 1642 |
| Publisher DOI: | 10.1292/jvms.21-0406 |
| Abstract: | We developed an analytical method using an on-line column-switching liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) for quantifying multiple steroids in serum. Using the developed method, we evaluated the serum concentration of nine steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 21-deoxycortisol, deoxycorticosterone, progesterone, 17 alpha-OH-progesterone and aldosterone) in dogs with hyperadrenocorticism ( HAC). Serum was mixed with stable isotope internal standards and thereafter purified by the automated column-switching system. The limit of detection ranged 2-16 pg/ml for nine steroids. In the baseline samples, five steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, and 17 alpha-OH-progesterone) were detected in all dogs. The concentrations of cortisone, 11-deoxycortisol, and 17 alpha-OH-progesterone in dogs with HAC (n=19) were significantly higher those in dogs without HAC (n=15, P<0.02). After the adrenocorticotropic hormone stimulation test, six steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 17 alpha-OH- progesterone, and deoxycorticosterone) were above the limit of quantification in all dogs. Cortisol, corticosterone, cortisone, and deoxycorticosterone concentrations of dogs with HAC were significantly higher than those of dogs without HAC (P<0.02). In addition, 11-deoxycortisol and 17 alpha-OH-progesterone concentration was higher in dogs with HAC than in dogs without HAC (P=0.044 and P=0.048, respectively). The on-line column-switching LC/MS/MS would be feasible for measuring multiple steroids in dog serum. The results suggest that cortisone, 11-deoxycortisol, and 17 alpha-OH-progesterone would be related to HAC. Further studies are warranted to assess the clinical feasibility of steroid profile in dogs with HAC. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/84327 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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