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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Peer-reviewed Journal Articles, etc >
Monitoring of chronic wasting disease using real-time quaking-induced conversion assay in Japan
| Title: | Monitoring of chronic wasting disease using real-time quaking-induced conversion assay in Japan |
| Authors: | Suzuki, Akio Browse this author | | Sawada, Kazuhei Browse this author | | Erdenebat, Temuulen Browse this author | | Yamasaki, Takeshi Browse this author | | Tobiume, Minoru Browse this author | | Suga, Kinuyo Browse this author | | Horiuchi, Motohiro Browse this author →KAKEN DB |
| Keywords: | chronic wasting disease | | prion | | prion protein | | real-time quaking-induced conversion | | scrapie |
| Issue Date: | 16-Nov-2021 |
| Publisher: | 公益社団法人 日本獣医学会 (The Japanese Society of Veterinary Science) |
| Journal Title: | Journal of veterinary medical science |
| Volume: | 83 |
| Issue: | 11 |
| Start Page: | 1735 |
| End Page: | 1739 |
| Publisher DOI: | 10.1292/jvms.21-0368 |
| Abstract: | There has been no report on Chronic wasting disease (CWD) cases in Japan to date; however, there is concern about the geographic spread of CWD. To clarify the CWD status in Japan, we conducted CWD monitoring using real-time quaking-induced conversion (RT-QuIC) assay which can detect the low level of CWD prions. A total of 690 obex samples collected from sika deer and Reeves's muntjac in Hokkaido and Honshu was tested for CWD prions. No CWD-positive cases were found, suggesting that CWD is nonexistent in Japan. Our results also indicate that RT-QuIC assay is useful for continuous monitoring of CWD. Furthermore, nucleotide sequence analysis of the PrP gene revealed sika deer in Japan harbor CWD susceptible allele. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/84329 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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