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Hokkaido University Collection of Scholarly and Academic Papers >
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Establishment of a mouse- and egg-adapted strain for the evaluation of vaccine potency against H3N2 variant influenza virus in mice
| Title: | Establishment of a mouse- and egg-adapted strain for the evaluation of vaccine potency against H3N2 variant influenza virus in mice |
| Authors: | Bazarragchaa, Enkhbold Browse this author | | Hiono, Takahiro Browse this author →KAKEN DB | | Isoda, Norikazu Browse this author →KAKEN DB | | Hayashi, Hirotaka Browse this author | | Okamatsu, Masatoshi Browse this author →KAKEN DB | | Sakoda, Yoshihiro Browse this author →KAKEN DB |
| Keywords: | adaptation | | H3N2 variant | | influenza | | mouse model | | vaccine |
| Issue Date: | 31-Oct-2021 |
| Publisher: | 公益社団法人 日本獣医学会 (The Japanese Society of Veterinary Science) |
| Journal Title: | Journal of veterinary medical science |
| Volume: | 83 |
| Issue: | 11 |
| Start Page: | 1694 |
| End Page: | 1701 |
| Publisher DOI: | 10.1292/jvms.21-0350 |
| Abstract: | Sporadic spreads of swine-origin influenza H3N2 variant (H3N2v) viruses were reported in humans, resulting in 437 human infections between 2011 and 2021 in the USA. Thus, an effective vaccine is needed to better control a potential pandemic for these antigenically distinct viruses from seasonal influenza. In this study, a candidate vaccine strain with efficient growth capacity in chicken embryos was established through serial blind passaging of A/Indiana/08/2011 (H3N2)v in mice and chicken embryos. Seven amino acid substitutions (M21I in PA; A138T, N165K, and V226A in HA; S312L in NP; T167I in M1; G62A in NS1 proteins) were found in the passaged viruses without a major change in the antigenicity. This mouse- and egg-adapted virus was used as a vaccine and challenge strain in mice to evaluate the efficacy of the H3N2v vaccine in different doses. Antibodies with high neutralizing titers were induced in mice immunized with 100 mu g of inactivated whole-virus particles, and those mice were significantly protected from the challenge of homologous strain. The findings indicated that the established strain in the study was useful for vaccine study in mouse models. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/84335 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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