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Dynamic Associations of Milk Components With the Infant Gut Microbiome and Fecal Metabolites in a Mother-Infant Model by Microbiome, NMR Metabolomic, and Time-Series Clustering Analyses

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Title: Dynamic Associations of Milk Components With the Infant Gut Microbiome and Fecal Metabolites in a Mother-Infant Model by Microbiome, NMR Metabolomic, and Time-Series Clustering Analyses
Authors: Komatsu, Yosuke Browse this author
Kumakura, Daiki Browse this author
Seto, Namiko Browse this author
Izumi, Hirohisa Browse this author
Takeda, Yasuhiro Browse this author
Ohnishi, Yuki Browse this author →KAKEN DB
Nakaoka, Shinji Browse this author →KAKEN DB
Aizawa, Tomoyasu Browse this author →KAKEN DB
Keywords: NMR metabolomics
time-series clustering analysis
breast milk
bioactive component
human milk oligosaccharide
infant gut microbiome
infant fecal metabolites
Issue Date: 7-Jan-2022
Publisher: Frontiers Media
Journal Title: Frontiers in Nutrition
Volume: 8
Start Page: 813690
Publisher DOI: 10.3389/fnut.2021.813690
Abstract: Background: The gut microbiome and fecal metabolites of breastfed infants changes during lactation, and are influenced by breast milk components. This study aimed to investigate dynamic associations of milk components with the infant gut microbiome and fecal metabolites throughout the lactation period in a mother-infant model.Methods: One month after delivery, breast milk and subsequent infant feces were collected in a pair for 5 months from a mother and an exclusively breastfed infant. Composition of the fecal microbiome was determined with 16S rRNA sequencing. Low-molecular-weight metabolites, including human milk oligosaccharides (HMOs), and antibacterial proteins were measured in feces and milk using H-1 NMR metabolomics and enzyme-linked immunosorbent assays. The association of milk bioactive components with the infant gut microbiome and fecal metabolites was determined with Python clustering and correlation analyses.Results: The HMOs in milk did not fluctuate throughout the lactation period. However, they began to disappear in infant feces at the beginning of month 4. Notably, at this time-point, a bifidobacterium species switching (from B. breve to B. longum subsp. infantis) occurred, accompanied by fluctuations in several metabolites including acetate and butyrate in infant feces.Conclusions: Milk bioactive components, such as HMOs, might play different roles in the exclusively breastfed infants depending on the lactation period.
Type: article
URI: http://hdl.handle.net/2115/84576
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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