| Title: | Identification of G protein-coupled receptor 55 (GPR55) as a target of curcumin |
| Authors: | Harada, Naoki Browse this author |
| Okuyama, Mai Browse this author |
| Teraoka, Yoshiaki Browse this author |
| Arahori, Yumi Browse this author |
| Shinmori, Yoh Browse this author |
| Horiuchi, Hiroko Browse this author |
| Luis, Paula B. Browse this author |
| Joseph, Akil I. Browse this author |
| Kitakaze, Tomoya Browse this author |
| Matsumura, Shigenobu Browse this author |
| Hira, Tohru Browse this author →KAKEN DB |
| Yamamoto, Norio Browse this author |
| Iuni, Takashi Browse this author |
| Goshima, Naoki Browse this author |
| Schneider, Claus Browse this author |
| Inui, Hiroshi Browse this author |
| Yamaji, Ryoichi Browse this author |
| Issue Date: | 2022 |
| Journal Title: | npj Science of Food |
| Volume: | 6 |
| Issue: | 1 |
| Start Page: | 4 |
| Publisher DOI: | 10.1038/s41538-021-00119-x |
| Abstract: | The identification of molecular targets of bioactive food components is important to understand the mechanistic aspect of their physiological functions. Here, we have developed a screening system that enables us to determine the activation of G protein-coupled receptors (GPCRs) by food components and have identified GPR55 as a target for curcumin. Curcumin activated GPR55 and induced serum-response element- and serum-response factor-mediated transcription, which were inhibited by Rho kinase and GPR55 antagonists. Both the methoxy group and the heptadienone moiety of curcumin were required for GPR55 activation. The F1905.47 residue of GPR55 was important for the interaction with curcumin. The curcumin-induced secretion of glucagon-like peptide-1 in GLUTag cells was inhibited by a GPR55 antagonist. These results indicate that expression screening is a useful system to identify GPCRs as targets of food components and strongly suggest that curcumin activates GPR55 as an agonist, which is involved in the physiological function of curcumin. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/84585 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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