|
|
Hokkaido University Collection of Scholarly and Academic Papers >
Theses >
博士 （理学） >
Molecular Evolutionary Studies on the Major Histocompatibility Complex of Japanese and Russian Raccoon Dogs, Nyctereutes procyonoides
|
Files in This Item:
|
|
|
Related Items in HUSCAP:
|
| Title: | Molecular Evolutionary Studies on the Major Histocompatibility Complex of Japanese and Russian Raccoon Dogs, Nyctereutes procyonoides |
| Other Titles: | 日本およびロシアにおけるタヌキ Nyctereutes procyonoides の主要組織適合遺伝子複合体に関する分子進化学的研究 |
| Authors: | Bartocillo, Aye Mee Forro Browse this author |
| Issue Date: | 25-Mar-2021 |
| Publisher: | Hokkaido University |
| Abstract: | Raccoon dogs, Nyctereutes procyonoides, are native to East Asia, but have been introduced into western Russian and eastern Europe. They have been reported to be affected by various pathogens causing diseases, endoparasites, and viruses. Nevertheless, these pathogens have not prevented the raccoon dog’s wide expansion in Far Eastern Asia and Europe. Recently, the major histocompatibility complex (MHC) genes have been widely analyzed to assess the immunological fitness and evolutionary adaptation in different populations, an essential key for conservation biology. Therefore, this study presents the allelic diversity and selection mechanism in the evolution of MHC class I and class II genes of Japanese and Russian raccoon dogs.
A total of 23 class II DRB alleles and a total of 48 novel MHC class I alleles were detected from 36 and 31 individuals of Japanese and Russian raccoon dogs, respectively. For both MHC class I and class II DRB, some alleles were found across the species’ range, while others were geographically restricted. Similarly, the ratio of non-synonymous to synonymous substitution rates for codons at the predicted antigen-binding sites for both classes were greater than 1, indicating that raccoon dog’s MHC class I and class II DRB genes have evolved under positive selection. Mixed effect model of evolution analysis and an algorithm to detect recombination showed positive selection sites at the amino acid level in both class I and class II molecules. Overall the results suggest that the diversity of MHC class I and class II DRB were influenced and maintained by recombination, pathogen-driven positive selection, and geographical barriers.
For MHC class II DRB, the Bayesian phylogenetic tree revealed no evidence of trans-species polymorphism (TSP), but instead showed the monophyletic relationships within the Canidae clade. The lack of TSP suggests a possible influence of species-specific pathogens driven by their environments and their long historical divergence from other canids. For MHC class I genes, Bayesian phylogenetic trees showed no evidence of TSP with alleles from carnivore species in other families, but did detect TSP between raccoon dogs and the domestic dog, indicative of long term balancing selection in canids.
This study is presented into two chapters: Chapter 1 provides the results and interpretation of the MHC class II genes of Japanese and Russian raccoon dogs, which is already published in the Biological Journal of the Linnean Society last September 10, 2019 with (https://doi.org/10.1093/biolinnean/blz153). While, Chapter 2 presents the results and interpretation of the MHC class I genes of Japanese and Russian raccoon dogs, which is currently under peer review. |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第14362号 |
| Degree Level: | 博士 |
| Degree Discipline: | 理学 |
| Examination Committee Members: | (主査) 教授 増田 隆一, 教授 髙木 昌興, 准教授 加藤 徹 |
| Degree Affiliation: | 理学院（自然史科学専攻） |
| Type: | theses (doctoral) |
| URI: | http://hdl.handle.net/2115/84671 |
| Appears in Collections: | 課程博士 (Doctorate by way of Advanced Course) > 理学院(Graduate School of Science)
学位論文 (Theses) > 博士 （理学）
|
|
