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Effect of Insertion and Deletion in the Meq Protein Encoded by Highly Oncogenic Marek's Disease Virus on Transactivation Activity and Virulence

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Title: Effect of Insertion and Deletion in the Meq Protein Encoded by Highly Oncogenic Marek's Disease Virus on Transactivation Activity and Virulence
Authors: Sato, Jumpei Browse this author
Murata, Shiro Browse this author →KAKEN DB
Yang, Zhiyuan Browse this author
Kaufer, Benedikt B. Browse this author
Fujisawa, Sotaro Browse this author
Seo, Hikari Browse this author
Maekawa, Naoya Browse this author →KAKEN DB
Okagawa, Tomohiro Browse this author →KAKEN DB
Konnai, Satoru Browse this author →KAKEN DB
Osterrieder, Nikolaus Browse this author
Parcells, Mark S. Browse this author
Ohashi, Kazuhiko Browse this author →KAKEN DB
Keywords: Marek's disease virus
Marek's disease
transactivation activity
Issue Date: Feb-2022
Publisher: MDPI
Journal Title: Viruses-Basel
Volume: 14
Issue: 2
Start Page: 382
End Page: 1
Publisher DOI: 10.3390/v14020382
Abstract: Marek's disease virus (MDV) causes malignant lymphoma in chickens (Marek's disease, MD). Although MD is currently controlled by vaccination, MDV strains have continuously increased in virulence over the recent decades. Polymorphisms in Meq, an MDV-encoded oncoprotein that serves as a transcription factor, have been associated with the enhanced virulence of the virus. In addition, insertions and deletions in Meq have been observed in MDV strains of higher virulence, but their contribution to said virulence remains elusive. In this study, we investigated the contribution of an insertion (L-Meq) and a deletion in the Meq gene (S-Meq) to its functions and MDV pathogenicity. Reporter assays revealed that both insertion and deletion enhanced the transactivation potential of Meq. Additionally, we generated RB-1B-based recombinant MDVs (rMDVs) encoding each Meq isoform and analyzed their pathogenic potential. rMDV encoding L-Meq indueced the highest mortality and tumor incidence in infected animals, whereas the rMDV encoding S-Meq exhibited the lowest pathogenicity. Thus, insertion enhanced the transactivation activity of Meq and MDV pathogenicity, whereas deletion reduced pathogenicity despite having increased transactivation activity. These data suggest that other functions of Meq affect MDV virulence. These data improve our understanding of the mechanisms underlying the evolution of MDV virulence.
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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