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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
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Effect of Insertion and Deletion in the Meq Protein Encoded by Highly Oncogenic Marek's Disease Virus on Transactivation Activity and Virulence
Title: | Effect of Insertion and Deletion in the Meq Protein Encoded by Highly Oncogenic Marek's Disease Virus on Transactivation Activity and Virulence |
Authors: | Sato, Jumpei Browse this author | Murata, Shiro Browse this author →KAKEN DB | Yang, Zhiyuan Browse this author | Kaufer, Benedikt B. Browse this author | Fujisawa, Sotaro Browse this author | Seo, Hikari Browse this author | Maekawa, Naoya Browse this author →KAKEN DB | Okagawa, Tomohiro Browse this author →KAKEN DB | Konnai, Satoru Browse this author →KAKEN DB | Osterrieder, Nikolaus Browse this author | Parcells, Mark S. Browse this author | Ohashi, Kazuhiko Browse this author →KAKEN DB |
Keywords: | Marek's disease virus | Marek's disease | Meq | CVI988 | tumorigenesis | pathogenicity | transactivation activity |
Issue Date: | Feb-2022 |
Publisher: | MDPI |
Journal Title: | Viruses-Basel |
Volume: | 14 |
Issue: | 2 |
Start Page: | 382 |
End Page: | 1 |
Publisher DOI: | 10.3390/v14020382 |
Abstract: | Marek's disease virus (MDV) causes malignant lymphoma in chickens (Marek's disease, MD). Although MD is currently controlled by vaccination, MDV strains have continuously increased in virulence over the recent decades. Polymorphisms in Meq, an MDV-encoded oncoprotein that serves as a transcription factor, have been associated with the enhanced virulence of the virus. In addition, insertions and deletions in Meq have been observed in MDV strains of higher virulence, but their contribution to said virulence remains elusive. In this study, we investigated the contribution of an insertion (L-Meq) and a deletion in the Meq gene (S-Meq) to its functions and MDV pathogenicity. Reporter assays revealed that both insertion and deletion enhanced the transactivation potential of Meq. Additionally, we generated RB-1B-based recombinant MDVs (rMDVs) encoding each Meq isoform and analyzed their pathogenic potential. rMDV encoding L-Meq indueced the highest mortality and tumor incidence in infected animals, whereas the rMDV encoding S-Meq exhibited the lowest pathogenicity. Thus, insertion enhanced the transactivation activity of Meq and MDV pathogenicity, whereas deletion reduced pathogenicity despite having increased transactivation activity. These data suggest that other functions of Meq affect MDV virulence. These data improve our understanding of the mechanisms underlying the evolution of MDV virulence. |
Type: | article |
URI: | http://hdl.handle.net/2115/84738 |
Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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