| Title: | Altered immunolocalization of FGF23 in murine femora metastasized with human breast carcinoma MDA-MB-231 cells |
| Authors: | Yokoyama, Ayako Browse this author |
| Hasegawa, Tomoka Browse this author →KAKEN DB |
| Hiraga, Toru Browse this author →KAKEN DB |
| Yamada, Tamaki Browse this author →KAKEN DB |
| Yimin Browse this author →KAKEN DB |
| Hongo, Hiromi Browse this author →KAKEN DB |
| Yamamoto, Tomomaya Browse this author |
| Abe, Miki Browse this author |
| Yoshida, Taiji Browse this author |
| Imanishi, Yasuo Browse this author →KAKEN DB |
| Kuroshima, Shinichiro Browse this author →KAKEN DB |
| Sasaki, Muneteru Browse this author →KAKEN DB |
| de Fraitas, Paulo Henrique Luiz Browse this author |
| Li, Minqi Browse this author |
| Amizuka, Norio Browse this author →KAKEN DB |
| Yamazaki, Yutaka Browse this author →KAKEN DB |
| Keywords: | Fibroblast growth factor 23 |
| Osteocyte |
| Immunohistochemistry |
| Bone metastasis |
| MDA-MB-231 |
| Issue Date: | 8-Apr-2021 |
| Publisher: | Springer |
| Journal Title: | Journal of Bone and Mineral Metabolism |
| Volume: | 39 |
| Issue: | 5 |
| Start Page: | 810 |
| End Page: | 823 |
| Publisher DOI: | 10.1007/s00774-021-01220-7 |
| PMID: | 33834310 |
| Abstract: | Introduction After the onset of bone metastasis, tumor cells appear to modify surrounding microenvironments for their benefit, and particularly, the levels of circulating fibroblast growth factor (FGF) 23 in patients with tumors have been highlighted. Materials and methods We have attempted to verify if human breast carcinoma MDA-MB-231 cells metastasized in the long bone of nu/nu mice would synthesize FGF23. Serum concentrations of calcium, phosphate (Pi) and FGF23 were measured in control nu/nu mice, bone-metastasized mice, and mice with mammary gland injected with MDA-MB-231 cells mimicking primary mammary tumors. Results and conclusions MDA-MB-231 cells revealed intense FGF23 reactivity in metastasized lesions, whereas MDA-MB-231 cells cultured in vitro or when injected into the mammary glands (without bone metastasis) showed weak FGF23 immunoreactivity. Although the bone-metastasized MDA-MB-231 cells abundantly synthesized FGF23, osteocytes adjacent to the FGF23-immunopositive tumors, unlike intact osteocytes, showed no FGF23. Despite significantly elevated serum FGF23 levels in bone-metastasized mice, there was no significant decrease in the serum Pi concentration when compared with the intact mice and mice with a mass of MDA-MB-231 cells in mammary glands. The metastasized femora showed increased expression and FGFR1 immunoreactivity in fibroblastic stromal cells, whereas femora of control mice showed no obvious FGFR1 immunoreactivity. Taken together, it seems likely that MDA-MB-231 cells synthesize FGF23 when metastasized to a bone, and thus affect FGFR1-positive stromal cells in the metastasized tumor nest in a paracrine manner. |
| Rights: | This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s00774-021-01220-7 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/84740 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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