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N-acetylcysteine attenuates PGE(2) and ROS production stimulated by 4-META/MMA-based resin in murine osteoblastic cells
Title: | N-acetylcysteine attenuates PGE(2) and ROS production stimulated by 4-META/MMA-based resin in murine osteoblastic cells |
Authors: | Nakamura, Koichi Browse this author →KAKEN DB | Minamikawa, Hajime Browse this author →KAKEN DB | Takahashi, Shizuka Browse this author | Yoshimura, Yoshitaka Browse this author →KAKEN DB | Yawaka, Yasutaka Browse this author →KAKEN DB |
Keywords: | 4-META/MMA-based resin | N-acetylcysteine | Reactive oxygen species |
Issue Date: | 29-May-2021 |
Publisher: | 日本歯科理工学会(The Japanese Society for Dental Materials and Devices) |
Journal Title: | Dental materials journal |
Volume: | 40 |
Issue: | 3 |
Start Page: | 808 |
End Page: | 812 |
Publisher DOI: | 10.4012/dmj.2020-275 |
PMID: | 33642448 |
Abstract: | This study examined the effects of N-acetylcysteine (NAC) on the inflammatory reactions of murine osteoblastic cells cultured on the 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate (4-META/MMA)-based resin. Superbond C&B (SB) was used as the 4-META/MMA-based resin and placed in a 48-well cell culture plate. The cells were cultured in alpha MEM (control) as well as on SB and SB in alpha MEM with NAC (SB+NAC). They were examined using the WST-1 proliferation assay, real-time PCR, enzyme-linked immunosorbent assay (ELISA), intracellular reactive oxygen species (ROS) measurements, and cellular glutathione (GSH) detection. COX-2 and IL-6 gene expressions were upregulated in SB; however, they were suppressed by NAC. Furthermore, PGE2 production in the culture medium was increased in SB, whereas NAC decreased the PGE(2) production. NAC lowered the ROS level in the culture medium and significantly increased the intracellular GSH level. The present in vitro study demonstrated that NAC might be effective for dental material detoxification. |
Type: | article |
URI: | http://hdl.handle.net/2115/85085 |
Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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