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N-acetylcysteine attenuates PGE(2) and ROS production stimulated by 4-META/MMA-based resin in murine osteoblastic cells

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Title: N-acetylcysteine attenuates PGE(2) and ROS production stimulated by 4-META/MMA-based resin in murine osteoblastic cells
Authors: Nakamura, Koichi Browse this author →KAKEN DB
Minamikawa, Hajime Browse this author →KAKEN DB
Takahashi, Shizuka Browse this author
Yoshimura, Yoshitaka Browse this author →KAKEN DB
Yawaka, Yasutaka Browse this author →KAKEN DB
Keywords: 4-META/MMA-based resin
Reactive oxygen species
Issue Date: 29-May-2021
Publisher: 日本歯科理工学会(The Japanese Society for Dental Materials and Devices)
Journal Title: Dental materials journal
Volume: 40
Issue: 3
Start Page: 808
End Page: 812
Publisher DOI: 10.4012/dmj.2020-275
PMID: 33642448
Abstract: This study examined the effects of N-acetylcysteine (NAC) on the inflammatory reactions of murine osteoblastic cells cultured on the 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate (4-META/MMA)-based resin. Superbond C&B (SB) was used as the 4-META/MMA-based resin and placed in a 48-well cell culture plate. The cells were cultured in alpha MEM (control) as well as on SB and SB in alpha MEM with NAC (SB+NAC). They were examined using the WST-1 proliferation assay, real-time PCR, enzyme-linked immunosorbent assay (ELISA), intracellular reactive oxygen species (ROS) measurements, and cellular glutathione (GSH) detection. COX-2 and IL-6 gene expressions were upregulated in SB; however, they were suppressed by NAC. Furthermore, PGE2 production in the culture medium was increased in SB, whereas NAC decreased the PGE(2) production. NAC lowered the ROS level in the culture medium and significantly increased the intracellular GSH level. The present in vitro study demonstrated that NAC might be effective for dental material detoxification.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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