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Proteolytic degradation of sardine (Sardinella gibbosa) proteins by trypsin from skipjack tuna (Katsuwonus pelamis) spleen
Title: | Proteolytic degradation of sardine (Sardinella gibbosa) proteins by trypsin from skipjack tuna (Katsuwonus pelamis) spleen |
Authors: | Klomklao, Sappasith Browse this author | Benjakul, Soottawat Browse this author | Visessanguan, Wonnop Browse this author | Kishimura, Hideki Browse this author →KAKEN DB | Simpson, Benjamin K. Browse this author |
Keywords: | Trypsin | Proteinase | Hydrolysis | Degradation | Myosin heavy chain | Muscle | Purification | Spleen | Tuna |
Issue Date: | 2006 |
Publisher: | Elsevier |
Journal Title: | Food Chemistry |
Volume: | 98 |
Issue: | 1 |
Start Page: | 14 |
End Page: | 22 |
Publisher DOI: | 10.1016/j.foodchem.2005.05.047 |
Abstract: | Trypsin from skipjack tuna (Katsuwonus pelamis) spleen was purified by ammonium sulfate precipitation and a series of chromatographies including Sephacryl S-100 and Benzamidine Sepharose 4 Fast Flow (high sub). The enzyme was purified to 22.3 folds with a yield of 51.6%. The molecular weight of trypsin was estimated to be 42 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Purified trypsin was able to hydrolyze natural actomyosin (NAM) and myosin, but rarely hydrolyzed collagen. Myosin heavy chain was most susceptible to hydrolysis by trypsin as evidenced by the lowest band intensity remained. The effect of NaCl on proteolytic activity was also studied. The band intensity of myosin heavy chain slightly increased as NaCl concentration increased, suggesting the inhibitory activity of NaCl. When hydrolytic activities of skipjack tuna spleen and bovine pancreas trypsins on sardine proteins, including NAM, myosin and collagen were compared, it was found that trypsin from bovine pancreas showed the greater activity towards NAM and myosin than that from skipjack tuna spleen. However, both enzymes could not degrade collagen. |
Relation: | http://www.sciencedirect.com/science/journal/03088146 |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/8509 |
Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 岸村 栄毅
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