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Genetic characterization of coliform bacterial isolates from environmental water in Thailand

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Title: Genetic characterization of coliform bacterial isolates from environmental water in Thailand
Authors: Tsunoda, Risa Browse this author
Usui, Masaru Browse this author
Tagaki, Chie Browse this author
Fukuda, Akira Browse this author
Boonla, Chanchai Browse this author
Anomasiri, Wilai Browse this author
Sukpanyatham, Nop Browse this author
Akapelwa, Mwangala Lonah Browse this author
Nakajima, Chie Browse this author →KAKEN DB
Tamura, Yutaka Browse this author
Suzuki, Yasuhiko Browse this author →KAKEN DB
Keywords: Enterobacteriaceae
IncFrepB plasmid
Issue Date: 1-May-2021
Publisher: Elsevier
Journal Title: Journal of infection and chemotherapy
Volume: 27
Issue: 5
Start Page: 722
End Page: 728
Publisher DOI: 10.1016/j.jiac.2020.12.023
Abstract: Introduction: In contrast to the study in other part of the world, information about characteristics of plasmids carrying antimicrobial resistance genes (ARGs) in Enterobacteriaceae derived from environmental water in tropical Asian countries including Thailand is limited. This study, therefore, aimed to gain insight into genetic information of antimicrobial resistance in environmental water in Thailand. Methods: Coliform bacteria were isolated from environmental water collected at 20 locations in Thailand and identified. Then, susceptibility profiles to ampicillin, cefazoline, cefotaxime, kanamycin, ciprofloxacin, sulfamethoxazole, tetracycline, and nalidixic acid were assessed. In addition, antimicrobial resistant genes integrons, and replicon types were analyzed. And furthermore, plasmids carrying bla(TEM) and tetM were identified by S1-PFGE analysis and confirmed transmissibility by transconjugation experiments. Results: In 130 coliform bacteria isolated, 89 were resistant to cefazoline while 41 isolates were susceptible. Cefazoline-resistant coliform bacteria were found to be significantly resistant to cefotaxime and tetracycline as compared to susceptible isolates. Hence, blaTEM and tetM correlating with beta-lactam antibiotics and tetracycline, respectively, were analyzed found to co-localize on the IncFrepB plasmids in isolates from pig farms' wastewater by S1-PFGE analysis. And furthermore, transmissibility of the plasmids was confirmed. Conclusions: Results obtained in this study suggested that ARGs in coliform bacteria may have been spreading on the farm via IncFrepB plasmids. Hence, appropriate use of antimicrobials and good hygiene management on the farm are required to prevent the emergence and spread of resistant bacteria. (C) 2021 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
Rights: © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鈴木 定彦

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