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Genetic characterization of coliform bacterial isolates from environmental water in Thailand
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| Title: | Genetic characterization of coliform bacterial isolates from environmental water in Thailand |
| Authors: | Tsunoda, Risa Browse this author | | Usui, Masaru Browse this author | | Tagaki, Chie Browse this author | | Fukuda, Akira Browse this author | | Boonla, Chanchai Browse this author | | Anomasiri, Wilai Browse this author | | Sukpanyatham, Nop Browse this author | | Akapelwa, Mwangala Lonah Browse this author | | Nakajima, Chie Browse this author →KAKEN DB | | Tamura, Yutaka Browse this author | | Suzuki, Yasuhiko Browse this author →KAKEN DB |
| Keywords: | Enterobacteriaceae | | Cefazoline-resistant | | IncFrepB plasmid | | bla(TEM) | | tetM |
| Issue Date: | 1-May-2021 |
| Publisher: | Elsevier |
| Journal Title: | Journal of infection and chemotherapy |
| Volume: | 27 |
| Issue: | 5 |
| Start Page: | 722 |
| End Page: | 728 |
| Publisher DOI: | 10.1016/j.jiac.2020.12.023 |
| Abstract: | Introduction: In contrast to the study in other part of the world, information about characteristics of plasmids carrying antimicrobial resistance genes (ARGs) in Enterobacteriaceae derived from environmental water in tropical Asian countries including Thailand is limited. This study, therefore, aimed to gain insight into genetic information of antimicrobial resistance in environmental water in Thailand. Methods: Coliform bacteria were isolated from environmental water collected at 20 locations in Thailand and identified. Then, susceptibility profiles to ampicillin, cefazoline, cefotaxime, kanamycin, ciprofloxacin, sulfamethoxazole, tetracycline, and nalidixic acid were assessed. In addition, antimicrobial resistant genes integrons, and replicon types were analyzed. And furthermore, plasmids carrying bla(TEM) and tetM were identified by S1-PFGE analysis and confirmed transmissibility by transconjugation experiments. Results: In 130 coliform bacteria isolated, 89 were resistant to cefazoline while 41 isolates were susceptible. Cefazoline-resistant coliform bacteria were found to be significantly resistant to cefotaxime and tetracycline as compared to susceptible isolates. Hence, blaTEM and tetM correlating with beta-lactam antibiotics and tetracycline, respectively, were analyzed found to co-localize on the IncFrepB plasmids in isolates from pig farms' wastewater by S1-PFGE analysis. And furthermore, transmissibility of the plasmids was confirmed. Conclusions: Results obtained in this study suggested that ARGs in coliform bacteria may have been spreading on the farm via IncFrepB plasmids. Hence, appropriate use of antimicrobials and good hygiene management on the farm are required to prevent the emergence and spread of resistant bacteria. (C) 2021 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved. |
| Rights: | © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/85103 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 鈴木 定彦
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