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Distribution and functional analysis of the two types of 8-vinyl reductase involved in chlorophyll biosynthesis in marine cyanobacteria
| Title: | Distribution and functional analysis of the two types of 8-vinyl reductase involved in chlorophyll biosynthesis in marine cyanobacteria |
| Authors: | Suehiro, Haruka Browse this author | | Tanaka, Ryouichi Browse this author →KAKEN DB | | Ito, Hisashi Browse this author |
| Keywords: | Synechococcus | | Acaryochloris | | 8-Vinyl reductase | | Chlorophyll biosynthesis | | Adaptation | | Low-iron environment |
| Issue Date: | 6-May-2021 |
| Publisher: | Springer |
| Journal Title: | Archives of Microbiology |
| Volume: | 203 |
| Start Page: | 3565 |
| End Page: | 3575 |
| Publisher DOI: | 10.1007/s00203-021-02348-w |
| Abstract: | In the chlorophyll biosynthesis pathway, the 8-vinyl group of the chlorophyll precursor is reduced to an ethyl group by 8-vinyl reductase. Two isozymes of 8-vinyl reductase have been described in oxygenic photosynthetic organisms: one encoded by BciA and another by BciB. Only BciB contains an [Fe-S] cluster and most cyanobacteria harbor this form; whereas a few contain BciA. Given this disparity in distribution, cyanobacterial BciA has remained largely overlooked, which has limited understanding of chlorophyll biosynthesis in these microorganisms. Here, we reveal that cyanobacterial BciA encodes a functional 8-vinyl reductase, as evidenced by measuring the in vitro activity of recombinant Synechococcus and Acaryochloris BciA. Genomic comparison revealed that BciB had been replaced by BciA during evolution of the marine cyanobacterium Synechococcus, and coincided with replacement of Fe-superoxide dismutase (SOD) with Ni-SOD. These findings imply that the acquisition of BciA confers an adaptive advantage to cyanobacteria living in low-iron oceanic environments. |
| Rights: | This is a post-peer-review, pre-copyedit version of an article published in Archives of Microbiology . The final authenticated version is available online at: http://dx.doi.org/10.1007/s00203-021-02348-w” |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/85106 |
| Appears in Collections: | 低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 伊藤 寿
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