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Characterization of a copper transporter 1 from Dermanyssus gallinae as a vaccine antigen

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Title: Characterization of a copper transporter 1 from Dermanyssus gallinae as a vaccine antigen
Authors: Fujisawa, Sotaro Browse this author
Murata, Shiro Browse this author →KAKEN DB
Isezaki, Masayoshi Browse this author
Ariizumi, Takuma Browse this author
Sato, Takumi Browse this author
Oishi, Eiji Browse this author
Taneno, Akira Browse this author
Maekawa, Naoya Browse this author →KAKEN DB
Okagawa, Tomohiro Browse this author →KAKEN DB
Ichii, Osamu Browse this author →KAKEN DB
Konnai, Satoru Browse this author →KAKEN DB
Ohashi, Kazuhiko Browse this author →KAKEN DB
Keywords: Copper transporter 1
Dermanyssus gallinae
poultry red mite
Issue Date: Jan-2022
Publisher: Cambridge University Press
Journal Title: Parasitology
Volume: 149
Issue: 1
Start Page: 105
End Page: 115
Publisher DOI: 10.1017/S0031182021001608
Abstract: Poultry red mites (Dermanyssus gallinae, PRM) are dangerous ectoparasites that infest chickens and threaten the poultry industry worldwide. PRMs usually develop resistance to chemical acaricides, necessitating the development of more effective preventive agents, and vaccination could be an alternative strategy for controlling PRMs. The suitability of plasma membrane proteins expressed in the midguts as vaccine antigens was evaluated because these molecules are exposed to antibodies in the ingested blood and the binding of antibodies could potentially induce direct damage to midgut tissue and indirect damage via inhibition of the functions of target molecules. Therefore, in the present study, a copper transporter 1-like molecule (Dg-Ctr1) was identified and its efficacy as a vaccine antigen was assessed in vitro. Dg-Ctr1 mRNA was expressed in the midguts and ovaries and in all the life stages, and flow cytometric analysis indicated that Dg-Ctr1 was expressed on the plasma membrane. Importantly, nymphs fed on plasma derived from chickens immunized with the recombinant protein of the extracellular region of Dg-Ctr1 showed a significant reduction in the survival rate. These data indicate that the application of Dg-Ctr1 as a vaccine antigen could reduce the number of nymphs in the farms, contributing to reduction in the economic losses caused by PRMs in the poultry industry. To establish an effective vaccination strategy, the acaricidal effects of the combined use of Dg-Ctr1 with chemical acaricides or other vaccine antigens must be examined.
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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