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Mapping of Antibody Epitopes on the Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein

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Title: Mapping of Antibody Epitopes on the Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein
Authors: Lombe, Boniface Pongombo Browse this author
Saito, Takeshi Browse this author
Miyamoto, Hiroko Browse this author
Mori-Kajihara, Akina Browse this author
Kajihara, Masahiro Browse this author
Saijo, Masayuki Browse this author
Masumu, Justin Browse this author
Hattori, Takanari Browse this author
Igarashi, Manabu Browse this author
Takada, Ayato Browse this author →KAKEN DB
Keywords: Crimean-Congo hemorrhagic fever virus
Nairobi sheep disease virus
monoclonal antibody
Issue Date: Mar-2022
Publisher: MDPI
Journal Title: Viruses-Basel
Volume: 14
Issue: 3
Start Page: 544
Publisher DOI: 10.3390/v14030544
Abstract: Crimean-Congo hemorrhagic fever virus (CCHFV), a nairovirus, is a tick-borne zoonotic virus that causes hemorrhagic fever in humans. The CCHFV nucleoprotein (NP) is the antigen most used for serological screening of CCHFV infection in animals and humans. To gain insights into antibody epitopes on the NP molecule, we produced recombinant chimeric NPs between CCHFV and Nairobi sheep disease virus (NSDV), which is another nairovirus, and tested rabbit and mouse antisera/immune ascites, anti-NP monoclonal antibodies, and CCHFV-infected animal/human sera for their reactivities to the NP antigens. We found that the amino acids at positions 161-320 might include dominant epitopes recognized by anti-CCHFV IgG antibodies, whereas cross-reactivity between anti-CCHFV and anti-NSDV antibodies was limited. Their binding capacities were further tested using a series of synthetic peptides whose sequences were derived from CCHFV NP. IgG antibodies in CCHFV-infected monkeys and patients were reactive to some of the synthetic peptide antigens (e.g., amino acid residues at positions 131-150 and 211-230). Only a few peptides were recognized by IgG antibodies in the anti-NSDV serum. These results provide useful information to improve NP-based antibody detection assays as well as antigen detection tests relying on anti-NP monoclonal antibodies.
Type: article
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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