|
Hokkaido University Collection of Scholarly and Academic Papers >
International Institute for Zoonosis Control >
Peer-reviewed Journal Articles, etc >
Mapping of Antibody Epitopes on the Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein
| Title: | Mapping of Antibody Epitopes on the Crimean-Congo Hemorrhagic Fever Virus Nucleoprotein |
| Authors: | Lombe, Boniface Pongombo Browse this author | | Saito, Takeshi Browse this author | | Miyamoto, Hiroko Browse this author | | Mori-Kajihara, Akina Browse this author | | Kajihara, Masahiro Browse this author | | Saijo, Masayuki Browse this author | | Masumu, Justin Browse this author | | Hattori, Takanari Browse this author | | Igarashi, Manabu Browse this author | | Takada, Ayato Browse this author →KAKEN DB |
| Keywords: | Crimean-Congo hemorrhagic fever virus | | CCHFV | | Nairobi sheep disease virus | | nucleoprotein | | antibody | | epitope | | monoclonal antibody |
| Issue Date: | Mar-2022 |
| Publisher: | MDPI |
| Journal Title: | Viruses-Basel |
| Volume: | 14 |
| Issue: | 3 |
| Start Page: | 544 |
| Publisher DOI: | 10.3390/v14030544 |
| Abstract: | Crimean-Congo hemorrhagic fever virus (CCHFV), a nairovirus, is a tick-borne zoonotic virus that causes hemorrhagic fever in humans. The CCHFV nucleoprotein (NP) is the antigen most used for serological screening of CCHFV infection in animals and humans. To gain insights into antibody epitopes on the NP molecule, we produced recombinant chimeric NPs between CCHFV and Nairobi sheep disease virus (NSDV), which is another nairovirus, and tested rabbit and mouse antisera/immune ascites, anti-NP monoclonal antibodies, and CCHFV-infected animal/human sera for their reactivities to the NP antigens. We found that the amino acids at positions 161-320 might include dominant epitopes recognized by anti-CCHFV IgG antibodies, whereas cross-reactivity between anti-CCHFV and anti-NSDV antibodies was limited. Their binding capacities were further tested using a series of synthetic peptides whose sequences were derived from CCHFV NP. IgG antibodies in CCHFV-infected monkeys and patients were reactive to some of the synthetic peptide antigens (e.g., amino acid residues at positions 131-150 and 211-230). Only a few peptides were recognized by IgG antibodies in the anti-NSDV serum. These results provide useful information to improve NP-based antibody detection assays as well as antigen detection tests relying on anti-NP monoclonal antibodies. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/85165 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|
