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Hokkaido University Collection of Scholarly and Academic Papers >
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Local free fatty acids trigger the expression of lipopolysaccharide-binding protein in murine white adipose tissue
| Title: | Local free fatty acids trigger the expression of lipopolysaccharide-binding protein in murine white adipose tissue |
| Authors: | Seki, Manami Browse this author | | Miwa, Akiho Browse this author | | Ohsaka, Fumina Browse this author →KAKEN DB | | Karatsu, Yugo Browse this author | | Tsuruta, Takeshi Browse this author | | Hino, Shingo Browse this author | | Morita, Tatsuya Browse this author | | Sonoyama, Kei Browse this author →KAKEN DB |
| Keywords: | lipopolysaccharide-binding protein | | high-fat diet | | white adipose tissue | | inflammation |
| Issue Date: | 2022 |
| Publisher: | BMFH Press |
| Journal Title: | Bioscience of Microbiota,Food And Health |
| Volume: | 41 |
| Issue: | 2 |
| Start Page: | 54 |
| End Page: | 65 |
| Publisher DOI: | 10.12938/bmfh.2021-061 |
| Abstract: | Although lipopolysaccharide (LPS)-binding protein (LBP) is an acute-phase protein mainly produced by hepatocytes, it has also been proposed to be a pro-inflammatory adipokine. Obesity and the consumption of a high-fat diet (HFD) are reportedly associated with elevated levels of LPS in plasma and free fatty acids (FFAs) in white adipose tissue (WAT). We examined whether circulating LPS or local FFAs are responsible for the HFD-induced increase of LBP in WAT. Male C57BL/6J mice were fed either a normal-fat diet (NFD) or an HFD. The mRNA levels in the liver and mesenteric WAT (mWAT), total FFA content in mWAT, and LBP and LPS concentrations in plasma were determined. The Lbp mRNA level in mWAT was higher in mice fed the HFD than in those fed the NFD for 3, 7, or 28 days or 14 weeks, whereas the hepatic Lbp mRNA level did not diller between the groups. The Lbp mRNA level in mWAT was also increased by the HFD in germ-free mice, which do not have gut microbiota, the source of LPS. The plasma LPS level did not show a significant correlation with the mWAT Lbp mRNA level. The total FFA content in mWAT was higher in mice fed the HFD than in those fed the NFD and positively correlated with the Lbp mRNA level. Supplementation with palmitic acid increased the Lbp mRNA level in 3T3-L1 adipocytes. We propose that local FFAs, but not circulating LPS, are the trigger for increased Lbp expression in mWAT of mice fed the HFD. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/85501 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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