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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
Antiadhesive nanosome elicits role of glycocalyx of tumor cell-derived exosomes in the organotropic cancer metastasis
| Title: | Antiadhesive nanosome elicits role of glycocalyx of tumor cell-derived exosomes in the organotropic cancer metastasis |
| Authors: | Koide, Ryosuke Browse this author | | Hirane, Nozomi Browse this author | | Kambe, Daiki Browse this author | | Yokoi, Yasuhiro Browse this author | | Otaki, Michiru Browse this author | | Nishimura, Shin-Ichiro Browse this author →KAKEN DB |
| Keywords: | Nanosome | | Exosome | | Glycocalyx | | Cancer metastasis | | Organotropism |
| Issue Date: | Jan-2022 |
| Publisher: | Elsevier |
| Journal Title: | Biomaterials |
| Volume: | 280 |
| Start Page: | 121314 |
| Publisher DOI: | 10.1016/j.biomaterials.2021.121314 |
| Abstract: | Despite emerging importance of tumor cells-derived exosomes in cancer metastasis, the heterogeneity of exosome populations has largely hampered systemic characterization of their molecular composition, biogenesis, and functions. This study communicates a novel method for predicting and targeting pre-metastatic sites based on an exosome model fluorescent cancer glyconanosomes displaying N-glycans of cultured tumor cells. Glycoblotting by antiadhesive quantum dots provides a nice tool to shed light on the pivotal functions of the glycocalyx reconstructed from four cancer cell types without bias due to other compositions of exosomes. In vivo imaging revealed that circulation, clearance, and organotropic biodistribution of cancer glyconanosomes in mice depend strongly on cancer cell-type-specific N-glycosylation patterns, the compositions of key glycotypes, particularly dominant abundances of high mannose-type N-glycans and the position-specific sialylation. Notably, organ biodistribution of cancer glyconanosomes is reproducible artificially by mimicking cancer cell-typespecific N-glycosylation patterns, demonstrating that nanosomal glycoblotting method serves as promising tools for predicting and targeting pre-metastatic sites determined by the glycocalyx of extracellular vesicles disseminated from the primary cancer site. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/85504 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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