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Vimentin-Ser82 as a memory phosphorylation site in astrocytes

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Title: Vimentin-Ser82 as a memory phosphorylation site in astrocytes
Authors: Oguri, Takashi Browse this author
Inoko, Akihito Browse this author
Shima, Hiroshi Browse this author
Izawa, Ichiro Browse this author
Arimura, Nariko Browse this author
Yamaguchi, Tomoya Browse this author
Inagaki, Naoyuki Browse this author
Kaibuchi, Kozo Browse this author
Kikuchi, Kunimi Browse this author
Inagaki, Masaki Browse this author
Keywords: protein-kinase-II
catalytic subunit
structural basis
Issue Date: May-2006
Publisher: Blackwell
Journal Title: Genes to cells
Volume: 11
Issue: 5
Start Page: 531
End Page: 540
Publisher DOI: 10.1111/j.1365-2443.2006.00961.x
Abstract: In astrocytes, the PGF(2 alpha) or ionomycin treatment induces the phosphorylation at Ser38 and Ser82 of vimentin, a type III intermediate filament, by Ca2+/calmodulin-dependent protein kinase II (CaMKII). We found here that vimentin phospho-Ser82 was dephosphorylated much slower than phospho-Ser38. Vimentin phospho-Ser38 was dephosphorylated quickly by purified PP1 catalytic subunit (PP1c) in vitro, whereas phospho-Ser82 was insensitive to PP1c. Because PP1c directly bound to vimentin through a VxF motif (Val83-Asp84-Phe85), the PP1c active site appeared to be unable to approach phospho-Ser82, leading to the prolongation of the phosphorylation at Ser-82. In astrocytes, PP1c alpha was in vivo associated with vimentin filaments. The repetitive treatment by ionomycin at a short interval resulted in the sustained elevation of Ser82 phosphorylation, leading to the marked disassembly of vimentin filaments. Taken together, these results suggest that vimentin is a novel member of binding partner of PP1c in astrocytes, and vimentin-Ser82 may act as a memory phosphorylation site.
Rights: Copyright c2006 by the Molecular Biology Society of Japan/Blaclwell Publishing
Type: article (author version)
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 菊池 九二三

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