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Vimentin-Ser82 as a memory phosphorylation site in astrocytes
| Title: | Vimentin-Ser82 as a memory phosphorylation site in astrocytes |
| Authors: | Oguri, Takashi Browse this author | | Inoko, Akihito Browse this author | | Shima, Hiroshi Browse this author | | Izawa, Ichiro Browse this author | | Arimura, Nariko Browse this author | | Yamaguchi, Tomoya Browse this author | | Inagaki, Naoyuki Browse this author | | Kaibuchi, Kozo Browse this author | | Kikuchi, Kunimi Browse this author | | Inagaki, Masaki Browse this author |
| Keywords: | protein-kinase-II | | intermediate-filaments | | catalytic subunit | | structural basis | | phosphatase-1 | | rat | | identification | | activation | | dynamics | | invitro |
| Issue Date: | May-2006 |
| Publisher: | Blackwell |
| Journal Title: | Genes to cells |
| Volume: | 11 |
| Issue: | 5 |
| Start Page: | 531 |
| End Page: | 540 |
| Publisher DOI: | 10.1111/j.1365-2443.2006.00961.x |
| Abstract: | In astrocytes, the PGF(2 alpha) or ionomycin treatment induces the phosphorylation at Ser38 and Ser82 of vimentin, a type III intermediate filament, by Ca2+/calmodulin-dependent protein kinase II (CaMKII). We found here that vimentin phospho-Ser82 was dephosphorylated much slower than phospho-Ser38. Vimentin phospho-Ser38 was dephosphorylated quickly by purified PP1 catalytic subunit (PP1c) in vitro, whereas phospho-Ser82 was insensitive to PP1c. Because PP1c directly bound to vimentin through a VxF motif (Val83-Asp84-Phe85), the PP1c active site appeared to be unable to approach phospho-Ser82, leading to the prolongation of the phosphorylation at Ser-82. In astrocytes, PP1c alpha was in vivo associated with vimentin filaments. The repetitive treatment by ionomycin at a short interval resulted in the sustained elevation of Ser82 phosphorylation, leading to the marked disassembly of vimentin filaments. Taken together, these results suggest that vimentin is a novel member of binding partner of PP1c in astrocytes, and vimentin-Ser82 may act as a memory phosphorylation site. |
| Rights: | Copyright c2006 by the Molecular Biology Society of Japan/Blaclwell Publishing |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/8555 |
| Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 菊池 九二三
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