| Title: | Ca2+ imaging with two-photon microscopy to detect the disruption of brain function in mice administered neonicotinoid insecticides |
| Authors: | Hirai, Anri Browse this author |
| Sugio, Shouta Browse this author |
| Nimako, Collins Browse this author |
| Nakayama, Shouta M. M. Browse this author →KAKEN DB |
| Kato, Keisuke Browse this author |
| Takahashi, Keisuke Browse this author |
| Arizono, Koji Browse this author |
| Hirano, Tetsushi Browse this author |
| Hoshi, Nobuhiko Browse this author |
| Fujioka, Kazutoshi Browse this author |
| Taira, Kumiko Browse this author |
| Ishizuka, Mayumi Browse this author →KAKEN DB |
| Wake, Hiroaki Browse this author |
| Ikenaka, Yoshinori Browse this author →KAKEN DB |
| Issue Date: | 24-Mar-2022 |
| Publisher: | Nature Portfolio |
| Journal Title: | Scientific reports |
| Volume: | 12 |
| Start Page: | 5114 |
| Publisher DOI: | 10.1038/s41598-022-09038-7 |
| Abstract: | Neonicotinoid pesticides are a class of insecticides that reportedly have harmful effects on bees and dragonflies, causing a reduction in their numbers. Neonicotinoids act as neuroreceptor modulators, and some studies have reported their association with neurodevelopmental disorders. However, the precise effect of neonicotinoids on the central nervous system has not yet been identified. Herein, we conducted in vivo Ca2+ imaging using a two-photon microscope to detect the abnormal activity of neuronal circuits in the brain after neonicotinoid application. The oral administration of acetamiprid (ACE) (20 mg/kg body weight (BW) in mature mice with a quantity less than the no-observed-adverse-effect level (NOAEL) and a tenth or half of the median lethal dose (LD50) of nicotine (0.33 or 1.65 mg/kg BW, respectively), as a typical nicotinic acetylcholine receptor (nAChR) agonist, increased anxiety-like behavior associated with altered activities of the neuronal population in the somatosensory cortex. Furthermore, we detected ACE and its metabolites in the brain, 1 h after ACE administration. The results suggested that in vivo Ca2+ imaging using a two-photon microscope enabled the highly sensitive detection of neurotoxicant-mediated brain disturbance of nerves. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/85600 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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