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Glu(333)in rabies virus glycoprotein is involved in virus attenuation through astrocyte infection and interferon responses

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Title: Glu(333)in rabies virus glycoprotein is involved in virus attenuation through astrocyte infection and interferon responses
Authors: Itakura, Yukari Browse this author
Tabata, Koshiro Browse this author
Morimoto, Kohei Browse this author
Ito, Naoto Browse this author
Chambaro, Herman M. Browse this author
Eguchi, Ryota Browse this author
Otsuguro, Ken-ichi Browse this author
Hall, William W. Browse this author
Orba, Yasuko Browse this author →KAKEN DB
Sawa, Hirofumi Browse this author →KAKEN DB
Sasaki, Michihito Browse this author →KAKEN DB
Issue Date: 15-Apr-2022
Publisher: Cell Press
Journal Title: iScience
Volume: 25
Issue: 4
Start Page: 104122
Publisher DOI: 10.1016/j.isci.2022.104122
Abstract: The amino add residue at position 333 of the rabies virus (RABV) glycoprotein (G333) is a major determinant of RABV pathogenicity. Virulent RABV strains possess Arg(333), whereas the attenuated strain HEP-Flury (HEP) possesses Glu(333). To investigate the potential attenuation mechanism dependent on a single amino add at G333, comparative analysis was performed between HEP and (HEPR)-R-333 mutant with Arg(333). We examined their respective tropism for astrocytes and the subsequent immune responses in astrocytes. Virus replication and subsequent interferon (IFN) responses in astrocytes infected with HEP were increased compared with (HEPR)-R-333 both in vitro and in vivo. Furthermore, involvement of IFN in the avirulency of HEP was demonstrated in IFN-receptor knockout mice. These results indicate that Glu(333) contributes to RABV attenuation by determining the ability of the virus to infect astrocytes and stimulate subsequent IFN responses.
Type: article
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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