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Requirement for expression of WW domain containing transcription regulator 1 in bovine trophectoderm development

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Title: Requirement for expression of WW domain containing transcription regulator 1 in bovine trophectoderm development
Authors: Saito, Shun Browse this author
Yamamura, Shota Browse this author
Kohri, Nanami Browse this author
Bai, Hanako Browse this author →KAKEN DB
Takahashi, Masashi Browse this author →KAKEN DB
Kawahara, Manabu Browse this author →KAKEN DB
Keywords: Embryo
Hippo pathway
Issue Date: 28-May-2021
Publisher: Elsevier
Journal Title: Biochemical and biophysical research communications
Volume: 555
Start Page: 140
End Page: 146
Publisher DOI: 10.1016/j.bbrc.2021.03.112
Abstract: WW domain-containing transcription regulator 1 (WWTR1) is one of the primary effectors in the Hippo pathway, which plays essential roles in cell differentiation into trophectoderm (TE) and inner cell mass cell lineages at the blastocyst stage. However, little is known about the roles of WWTR1 in preimplantation development. The present study aimed to explore the significance of WWTR1 expression in preimplantation development using an mRNA knockdown (KD) system in bovine embryos. We first quantitated WWTR1 expression at protein and mRNA levels from fertilization to blastocyst stage. WWTR1 proteins gradually shifted from extranuclear localization during the 16-cell stage to nuclear localization by morula stage. WWTR1 mRNA expression was also transiently upregulated at the 16-cell stage. WWTR1 KD efficiently repressed WWTR1 expression at protein and mRNA levels. The WWTR1 KD embryos developed to the blastocyst stage at rates equivalent to those of controls, but TE cell numbers were significantly decreased. Representative TE-expressed genes, including CDX2 and IFNT were also significantly decreased in WWTR1 KD blastocysts. These results provide the first demonstration that WWTR1 expression is responsible for normal TE cell development in preimplantation embryos. (c) 2021 Elsevier Inc. All rights reserved.
Rights: © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川原 学

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