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TSS-seq of Toxoplasma gondii sporozoites revealed a novel motif in stage-specific promoters

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Title: TSS-seq of Toxoplasma gondii sporozoites revealed a novel motif in stage-specific promoters
Authors: Kidaka, Taishi Browse this author
Sugi, Tatsuki Browse this author
Hayashida, Kyoko Browse this author
Suzuki, Yutaka Browse this author
Xuan, Xuenan Browse this author
Dubey, Jitender P. Browse this author
Yamagishi, Junya Browse this author →KAKEN DB
Keywords: Toxoplasma gondii
Transcriptional start sites
Issue Date: Mar-2022
Publisher: Elsevier
Journal Title: Infection, Genetics and Evolution
Volume: 98
Start Page: 105213
Publisher DOI: 10.1016/j.meegid.2022.105213
Abstract: Toxoplasma gondii is one of the most common zoonotic protozoan parasites. It has three major infectious stages: rapidly multiplying tachyzoites (Tz), slowly replicating bradyzoites (Bz) and a resting/free-living stage, sporo-zoites (Sz). The regulatory mechanisms governing stage-specific gene expression are not fully understood. Few transcriptional start sites (TSS) are known for Sz. In this study, we obtained TSS of Sz using an oligo-capping method and RNA-seq analysis. We identified 1,043,503 TSS in the Sz transcriptome. These defined 38,973 TSS clusters, of which, 11,925 were expressed in Sz and 1535 TSS differentially expressed in Sz. Based on these data, we defined promoter regions and novel sporozoite stage-specific motifs using MEME. TGTANNTACA was distributed around-55 to-75 regions from each TSS. Interestingly, the same motif was reported in another apicomplexan, Plasmodium berghei, as a cis-element of female-specific gametocyte genes, implying the presence of common regulatory machinery. Further comparative analysis should better define the distribution and function of these elements in other members of this important parasitic phylum.
Type: article
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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