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Alpha and beta adrenoceptors activate interleukin-6 transcription through different pathways in cultured astrocytes from rat spinal cord
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| Title: | Alpha and beta adrenoceptors activate interleukin-6 transcription through different pathways in cultured astrocytes from rat spinal cord |
| Authors: | Morimoto, Kohei Browse this author | | Eguchi, Ryota Browse this author →KAKEN DB | | Kitano, Taisuke Browse this author | | Otsuguro, Ken-ichi Browse this author →KAKEN DB |
| Keywords: | Astrocyte | | Noradrenaline | | Adrenoceptor | | Interleukin-6 | | MAPK |
| Issue Date: | 1-Jun-2021 |
| Publisher: | Elsevier |
| Journal Title: | Cytokine |
| Volume: | 142 |
| Start Page: | 155497 |
| Publisher DOI: | 10.1016/j.cyto.2021.155497 |
| Abstract: | In brain astrocytes, noradrenaline (NA) has been shown to up-regulate IL-6 production via 13-adrenoceptors (ARs). However, the underlying intracellular mechanisms for this regulation are not clear, and it remains unknown whether ?-ARs are involved. In this study, we investigated the AR-mediated regulation of IL-6 mRNA levels in the cultured astrocytes from rat spinal cord. NA, the ?1-agonist phenylephrine, and the 13-agonist isoproterenol increased IL-6 mRNA levels. The phenylephrine-induced IL-6 increase was accompanied by an increase in ERK phosphorylation, and these effects were blocked by inhibitors of PKC and ERK. The isoproterenol-induced IL-6 increase was accompanied by an increase in CREB phosphorylation, and these effects were blocked by a PKA inhibitor. Our results indicate that IL-6 increases by ?1- and 13-ARs are mediated via the PKC/ERK and cAMP/PKA/CREB pathways, respectively. Moreover, conditioned medium collected from astrocytes treated with the ?2-AR agonist dexmedetomidine, increased IL-6 mRNA in other astrocytes. In this study, we elucidate that ?1- and ?2-ARs, in addition to 13-ARs, promote IL-6 transcription through different pathways in spinal cord astrocytes. |
| Rights: | © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/85642 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 乙黒 兼一
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