| Title: | Prostaglandin-related immune suppression in cattle |
| Authors: | Sajiki, Y. Browse this author |
| Konnai, S. Browse this author |
| Ikenaka, Y. Browse this author |
| Okagawa, T. Browse this author |
| Maekawa, N. Browse this author |
| Logullo, C. Browse this author |
| Vaz Jr, I. da Silva Browse this author |
| Murata, S. Browse this author |
| Ohashi, K. Browse this author |
| Keywords: | Cattle |
| Immunosuppression |
| Prostaglandin |
| Prostaglandin A(1) |
| Th1 response |
| Issue Date: | Jun-2021 |
| Publisher: | Elsevier |
| Journal Title: | Veterinary Immunology and Immunopathology |
| Volume: | 236 |
| Start Page: | 110238 |
| Publisher DOI: | 10.1016/j.vetimm.2021.110238 |
| Abstract: | Prostaglandins (PGs) are lipid mediators derived from arachidonic acid by several enzymes including cyclooxygenase (COX)-1 and COX-2. We have previously shown that PGE2 regulates immune responses, such as Th1 cytokine production and T-cell proliferation, in cattle. However, it is still unclear whether other PGs are involved in the regulation of immune responses in cattle. Here, immunosuppressive profiles of PGs (PGA(1), PGB(2), PGD(2), PGE(2), PGF(1 alpha) and PGF(2 alpha)) were firstly examined using bovine peripheral blood mononuclear cells (PBMCs). In addition to PGE(2), PGA(1) significantly inhibited Th1 cytokine production from PBMCs in cattle. Further analyses focusing on PGA(1) revealed that treatment with PGA(1) in the presence of concanavalin A (con A) downregulated CD69, an activation marker, and IFN-gamma expression in both CD4(+) and CD8(+) T cells. Sorted CD3(+) T cells stimulated with con A were cultivated with PGA1, and IFN-gamma and TNF-alpha concentrations decreased upon PGA(1) treatment. Taken together, these results suggest that the treatment with PGA(1) in vitro inhibits T-cell activation, especially Th1 cytokine production, in cattle. |
| Rights: | ©2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/85643 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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