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A GCDGC-specific DNA (cytosine-5) methyltransferase that methylates the GCWGC sequence on both strands and the GCSGC sequence on one strand

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Title: A GCDGC-specific DNA (cytosine-5) methyltransferase that methylates the GCWGC sequence on both strands and the GCSGC sequence on one strand
Authors: Furuta, Yoshikazu Browse this author
Miura, Fumihito Browse this author
Ichise, Takahiro Browse this author
Nakayama, Shouta M. M. Browse this author →KAKEN DB
Ikenaka, Yoshinori Browse this author →KAKEN DB
Zorigt, Tuvshinzaya Browse this author
Tsujinouchi, Mai Browse this author
Ishizuka, Mayumi Browse this author →KAKEN DB
Ito, Takashi Browse this author
Higashi, Hideaki Browse this author →KAKEN DB
Issue Date: 21-Mar-2022
Publisher: PLOS
Journal Title: PLoS ONE
Volume: 17
Issue: 3
Start Page: e0265225
Publisher DOI: 10.1371/journal.pone.0265225
Abstract: 5-Methylcytosine is one of the major epigenetic marks of DNA in living organisms. Some bacterial species possess DNA methyltransferases that modify cytosines on both strands to produce fully-methylated sites or on either strand to produce hemi-methylated sites. In this study, we characterized a DNA methyltransferase that produces two sequences with different methylation patterns: one methylated on both strands and another on one strand. M.BatI is the orphan DNA methyltransferase of Bacillus anthracis coded in one of the prophages on the chromosome. Analysis of M.BatI modified DNA by bisulfite sequencing revealed that the enzyme methylates the first cytosine in sequences of 5ʹ-GCAGC-3ʹ, 5ʹ-GCTGC-3ʹ, and 5ʹ-GCGGC-3ʹ, but not of 5ʹ-GCCGC-3ʹ. This resulted in the production of fully-methylated 5ʹ-GCWGC-3ʹ and hemi-methylated 5ʹ-GCSGC-3ʹ. M.BatI also showed toxicity when expressed in E. coli, which was caused by a mechanism other than DNA modification activity. Homologs of M.BatI were found in other Bacillus species on different prophage like regions, suggesting the spread of the gene by several different phages. The discovery of the DNA methyltransferase with unique modification target specificity suggested unrevealed diversity of target sequences of bacterial cytosine DNA methyltransferase.
Type: article
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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