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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
1H NMR metabolomic and transcriptomic analyses reveal urinary metabolites as biomarker candidates in response to protein undernutrition in adult rats
| Title: | 1H NMR metabolomic and transcriptomic analyses reveal urinary metabolites as biomarker candidates in response to protein undernutrition in adult rats |
| Authors: | Komatsu, Yosuke Browse this author | | Wada, Yasuaki Browse this author | | Izumi, Hirohisa Browse this author | | Shimizu, Takashi Browse this author | | Takeda, Yasuhiro Browse this author | | Aizawa, Tomoyasu Browse this author →KAKEN DB |
| Keywords: | Metabolomics | | Multivariate analysis | | Protein undernutrition | | Transcriptomics | | Urinary metabolites |
| Issue Date: | 20-Aug-2020 |
| Publisher: | Cambridge University Press |
| Journal Title: | British Journal of Nutrition |
| Volume: | 125 |
| Issue: | 6 |
| Start Page: | 633 |
| End Page: | 643 |
| Publisher DOI: | 10.1017/S0007114520003281 |
| Abstract: | Protein undernutrition contributes to the development of various diseases in broad generations. Urinary metabolites may serve as non-invasive biomarkers of protein undernutrition; however, this requires further investigation. We aimed to identify novel urinary metabolites as biomarker candidates responsive to protein undernutrition. Adult rats were fed control (CT; 14 % casein) or isoenergetic low-protein (LP; 5 % casein) diets for 4 weeks. 1H NMR metabolomics was applied to urine, plasma and liver samples to identify metabolites responsive to protein undernutrition. Liver samples were subjected to mRNA microarray and quantitative PCR analyses to elucidate the mechanisms causing fluctuations in identified metabolites. Urinary taurine levels were significantly lower in the LP group than in the CT group at week 1 and remained constant until week 4. Hepatic taurine level and gene expression level of cysteine dioxygenase type 1 were also significantly lower in the LP group than in the CT group. Urinary trimethylamine N-oxide (TMAO) levels were significantly higher in the LP group than in the CT group at week 2 and remained constant until week 4. Hepatic TMAO level and gene expression levels of flavin-containing mono-oxygenase 1 and 5 were also significantly higher in the LP group than in the CT group. In conclusion, urinary taurine and TMAO levels substantially responded to protein undernutrition. Furthermore, changes in hepatic levels of these metabolites and gene expressions associated with their metabolic pathways were also reflected in their fluctuating urinary levels. Thus, taurine and TMAO could act as non-invasive urinary biomarker candidates to detect protein undernutrition. |
| Rights: | © The Author(s), 2020. Published by Cambridge University Press on behalf of The Nutrition Society |
| Type: | article |
| URI: | http://hdl.handle.net/2115/86311 |
| Appears in Collections: | 国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 相沢 智康
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