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Heat stress induces oxidative stress and activates the KEAP1-NFE2L2-ARE pathway in bovine endometrial epithelial cells

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Title: Heat stress induces oxidative stress and activates the KEAP1-NFE2L2-ARE pathway in bovine endometrial epithelial cells
Authors: Murata, Hirona Browse this author
Kunii, Hiroki Browse this author
Kusama, Kazuya Browse this author
Sakurai, Toshihiro Browse this author
Bai, Hanako Browse this author →KAKEN DB
Kawahara, Manabu Browse this author →KAKEN DB
Takahashi, Masashi Browse this author →KAKEN DB
Keywords: bovine uterus
endometrial epithelial cells
heat stress
oxidative stress
Issue Date: 22-Jul-2021
Publisher: Oxford University Press
Journal Title: Biology of reproduction
Volume: 105
Issue: 5
Start Page: 1114
End Page: 1125
Publisher DOI: 10.1093/biolre/ioab143
Abstract: Heat stress adversely affects the reproductive function in cows. Although a relationship between heat stress and oxidative stress has been suggested, it has not been sufficiently verified in bovine endometrial epithelial cells. Here, we investigated whether oxidative stress is induced by heat stress in bovine endometrial epithelial cells under high temperature. Luciferase reporter assays showed that the reporter activity of heat shock element and antioxidant responsive element was increased in endometrial epithelial cells cultured under high temperature compared to that in cells cultured under basal (thermoneutral) temperature. Also, nuclear factor, erythroid 2 like 2 (NFE2L2), a master regulator of cellular environmental stress response, stabilized and the expression levels of antioxidant enzyme genes increased under high temperature. Immunostaining confirmed the nuclear localization of NFE2L2 in endometrial epithelial cells cultured under high temperature. Quantitative polymerase chain reaction analysis showed that the expression levels of representative inflammatory cytokine genes, such as prostaglandin-endoperoxide synthase 2 (PTGS2) and interleukin 8, were significantly decreased in endometrial epithelial cells cultured under high temperature compared to those in cells cultured under basal temperature. Thus, our results suggest that heat stress induces oxidative stress, whereas NFE2L2 plays a protective role in bovine endometrial epithelial cells cultured under heat stress conditions. Summary sentence Heat stress induces oxidative stress, whereas NFE2L2, a master regulator of the cellular environmental stress response, has a protective role in bovine endometrial epithelial cells cultured under heat stress conditions.
Rights: This is a pre-copyedited, author-produced version of an article accepted for publication in Biology of reproduction following peer review. The version of record Hirona Murata, Hiroki Kunii, Kazuya Kusama, Toshihiro Sakurai, Hanako Bai, Manabu Kawahara, Masashi Takahashi, Heat stress induces oxidative stress and activates the KEAP1-NFE2L2-ARE pathway in bovine endometrial epithelial cells, Biology of Reproduction, Volume 105, Issue 5, November 2021, Pages 1114-1125 is available online at: https://doi.org/10.1093/biolre/ioab143.
Type: article (author version)
URI: http://hdl.handle.net/2115/86365
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 唄 花子

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