|
Hokkaido University Collection of Scholarly and Academic Papers >
International Institute for Zoonosis Control >
Peer-reviewed Journal Articles, etc >
Drug-resistant Mycobacterium tuberculosis and its genotypes isolated from an outbreak in western Thailand
| Title: | Drug-resistant Mycobacterium tuberculosis and its genotypes isolated from an outbreak in western Thailand |
| Authors: | Rudeeaneksin, Janisara Browse this author | | Phetsuksiri, Benjawan Browse this author | | Nakajima, Chie Browse this author →KAKEN DB | | Bunchoo, Supranee Browse this author | | Suthum, Krairerk Browse this author | | Tipkrua, Nattakan Browse this author | | Fukushima, Yukari Browse this author | | Suzuki, Yasuhiko Browse this author →KAKEN DB |
| Keywords: | MIRU-VNTR | | mutation | | outbreak | | spoligotyping | | TB | | Thailand |
| Issue Date: | 1-Aug-2021 |
| Publisher: | Oxford University Press |
| Journal Title: | Transactions of the Royal Society of Tropical Medicine and Hygiene |
| Volume: | 115 |
| Issue: | 8 |
| Start Page: | 886 |
| End Page: | 895 |
| Publisher DOI: | 10.1093/trstmh/traa148 |
| Abstract: | Background: Multidrug-resistant TB (MDR-TB) outbreaks have occurred in the Thamaka district, Kanchanaburi province in Thailand. Methods: Seventy-two isolates, which included 7% mono-, 30.6% MDR and extensively drug-resistant TB (XDR-TB), were genotyped by spoligotyping, mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) and single nucleotide polymorphism genotyping, and their drug resistance was analysed. Results: The spoligotyping results showed that Beijing spoligo-international type (SIT)1 was predominant (n=38; 52.8%) while the remaining were non-Beijing sublineages (n=34). The MIRU-VNTR analysis showed that Beijing isolates, most of which belonged to the modern type (n=37), formed 5 clusters and 13 individual patterns. In katG, only mutation Ser315Thr was identified. In rpoB, Ser531Leu was predominant, except for His526Arg and Leu533Pro, which were found in two isolates. A cluster of 14 Beijing strains contained these common mutations and shared the MIRU-VNTR genotype with isolates in the Thamaka district that had spread previously. Two U SIT523 isolates contained the mutations A1400G in rrs and Asp94Gly in gyrA genes, indicating a spread of XDR-TB. Conclusions: Most mutations were associated with drug resistance and the specific MDR Beijing and XDR-TB in U SIT523 isolates remain. This genotyping is a key tool for tracking TB transmission in the Thamaka district of Thailand. |
| Rights: | This is a pre-copyedited, author-produced version of an article accepted for publication in Transactions of the Royal Society of Tropical Medicine and Hygiene following peer review. The version of record is available online at:http://doi.org/10.1093/trstmh/traa148. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/86428 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 鈴木 定彦
|
