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Drug-resistant Mycobacterium tuberculosis and its genotypes isolated from an outbreak in western Thailand

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Title: Drug-resistant Mycobacterium tuberculosis and its genotypes isolated from an outbreak in western Thailand
Authors: Rudeeaneksin, Janisara Browse this author
Phetsuksiri, Benjawan Browse this author
Nakajima, Chie Browse this author →KAKEN DB
Bunchoo, Supranee Browse this author
Suthum, Krairerk Browse this author
Tipkrua, Nattakan Browse this author
Fukushima, Yukari Browse this author
Suzuki, Yasuhiko Browse this author →KAKEN DB
Keywords: MIRU-VNTR
mutation
outbreak
spoligotyping
TB
Thailand
Issue Date: 1-Aug-2021
Publisher: Oxford University Press
Journal Title: Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume: 115
Issue: 8
Start Page: 886
End Page: 895
Publisher DOI: 10.1093/trstmh/traa148
Abstract: Background: Multidrug-resistant TB (MDR-TB) outbreaks have occurred in the Thamaka district, Kanchanaburi province in Thailand. Methods: Seventy-two isolates, which included 7% mono-, 30.6% MDR and extensively drug-resistant TB (XDR-TB), were genotyped by spoligotyping, mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR) and single nucleotide polymorphism genotyping, and their drug resistance was analysed. Results: The spoligotyping results showed that Beijing spoligo-international type (SIT)1 was predominant (n=38; 52.8%) while the remaining were non-Beijing sublineages (n=34). The MIRU-VNTR analysis showed that Beijing isolates, most of which belonged to the modern type (n=37), formed 5 clusters and 13 individual patterns. In katG, only mutation Ser315Thr was identified. In rpoB, Ser531Leu was predominant, except for His526Arg and Leu533Pro, which were found in two isolates. A cluster of 14 Beijing strains contained these common mutations and shared the MIRU-VNTR genotype with isolates in the Thamaka district that had spread previously. Two U SIT523 isolates contained the mutations A1400G in rrs and Asp94Gly in gyrA genes, indicating a spread of XDR-TB. Conclusions: Most mutations were associated with drug resistance and the specific MDR Beijing and XDR-TB in U SIT523 isolates remain. This genotyping is a key tool for tracking TB transmission in the Thamaka district of Thailand.
Rights: This is a pre-copyedited, author-produced version of an article accepted for publication in Transactions of the Royal Society of Tropical Medicine and Hygiene following peer review. The version of record is available online at:http://doi.org/10.1093/trstmh/traa148.
Type: article
URI: http://hdl.handle.net/2115/86428
Appears in Collections:人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鈴木 定彦

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