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Antiviral Effects of 5-Aminolevulinic Acid Phosphate against Classical Swine Fever Virus : In Vitro and In Vivo Evaluation

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Title: Antiviral Effects of 5-Aminolevulinic Acid Phosphate against Classical Swine Fever Virus : In Vitro and In Vivo Evaluation
Authors: Hirose, Shizuka Browse this author
Isoda, Norikazu Browse this author →KAKEN DB
Huynh, Loc Tan Browse this author
Kim, Taksoo Browse this author
Yoshimoto, Keiichiro Browse this author
Tanaka, Tohru Browse this author
Inui, Kenjiro Browse this author
Hiono, Takahiro Browse this author →KAKEN DB
Sakoda, Yoshihiro Browse this author →KAKEN DB
Keywords: 5-aminolevulinic acid phosphate
antiviral effect
classical swine fever virus
protoporphyrin IX
Issue Date: 27-Jan-2022
Publisher: MDPI
Journal Title: Pathogens
Volume: 11
Issue: 2
Start Page: 164
Publisher DOI: 10.3390/pathogens11020164
Abstract: The inhibitory effects of 5-aminolevulinic acid phosphate (5-ALA), an important amino acid for energy production in the host, against viral infections were previously reported. Here, the antiviral effects of 5-ALA against classical swine fever virus (CSFV) belonging to the genus Pestivirus in the Flaviviridae family and its possible mechanisms were investigated. CSFV replication was suppressed in swine cells supplemented with 5-ALA or its metabolite, protoporphyrin IX (PPIX). The infectivity titer of CSFV was decreased after mixing with PPIX extracellularly. In addition, the activities of the replication cycle were decreased in the presence of PPIX based on the CSFV replicon assay. These results showed that PPIX exerted antiviral effects by inactivating virus particles and inhibiting the replication cycle. To evaluate the in vivo efficacy of 5-ALA, pigs were supplemented daily with 5-ALA for 1 week before virus inoculation and then inoculated with a virulent CSFV strain at the 10(7.0) 50% tissue culture infectious dose. The clinical scores of the supplemented group were significantly lower than those of the nonsupplemented group, whereas the virus growth was not. Taken together, 5-ALA showed antiviral effects against CSFV in vitro, and PPIX played a key role by inactivating virus particles extracellularly and inhibiting the replication cycle intracellularly.
Type: article
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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