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Taurocholic acid, a primary 12 alpha-hydroxylated bile acid, induces leakiness in the distal small intestine in rats

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Title: Taurocholic acid, a primary 12 alpha-hydroxylated bile acid, induces leakiness in the distal small intestine in rats
Authors: Liu, Hongxia Browse this author
Kohmoto, Ohji Browse this author
Sakaguchi, Ayana Browse this author
Hori, Shota Browse this author
Tochigi, Misuzu Browse this author
Tada, Koji Browse this author
Lee, Yeonmi Browse this author
Kikuchi, Keidai Browse this author
Ishizuka, Satoshi Browse this author →KAKEN DB
Keywords: Bile acid
Enterohepatic circulation
Gut permeability
Barrier function
Issue Date: Jul-2022
Publisher: Elsevier
Journal Title: Food and chemical toxicology
Volume: 165
Start Page: 113136
Publisher DOI: 10.1016/j.fct.2022.113136
Abstract: A high-fat diet increases 12 alpha-hydroxylated (12 alpha OH) bile acid (BA) secretion in rats, and secondary BAs are responsible for the leaky gut. This study aimed to examine the role of primary 12 alpha OH BAs in gut barrier impairment in rats using dietary cholic acid (CA) supplementation (0.5 g/kg diet). The CA diet increased the 12 alpha OH BAs concentrations in the small and large intestine, accompanied by gut barrier impairment. Based on the luminal 12 alpha OH BAs concentrations, ex vivo gut leakiness was determined. Deoxycholic acid increased permeability in the large intestine, whereas taurocholic acid (TCA) increased the ileal permeability, but not jejunal permeability. A Rho kinase inhibitor attenuated TCA-induced ileal permeability. Administration of vancomycin, which abolishes secondary BAs, did not influence the gut leakiness induced by the CA diet. Changes in the gut permeation marker in the tail vein blood suggested the possibility that the CA-induced leakiness occurred in the small intestine. The CA diet enhanced the phosphorylation of myosin light chain 2 and reduced claudins expressions in rat ileal epithelia. Reductions in barrier function-related genes were observed in the ileum, but not in the colon of the CA-fed rats. Overall, the present study demonstrated the significance of TCA in proximal gut leakiness.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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