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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Taurocholic acid, a primary 12 alpha-hydroxylated bile acid, induces leakiness in the distal small intestine in rats
| Title: | Taurocholic acid, a primary 12 alpha-hydroxylated bile acid, induces leakiness in the distal small intestine in rats |
| Authors: | Liu, Hongxia Browse this author | | Kohmoto, Ohji Browse this author | | Sakaguchi, Ayana Browse this author | | Hori, Shota Browse this author | | Tochigi, Misuzu Browse this author | | Tada, Koji Browse this author | | Lee, Yeonmi Browse this author | | Kikuchi, Keidai Browse this author | | Ishizuka, Satoshi Browse this author →KAKEN DB |
| Keywords: | Bile acid | | Enterohepatic circulation | | Ileum | | Gut permeability | | Barrier function |
| Issue Date: | Jul-2022 |
| Publisher: | Elsevier |
| Journal Title: | Food and chemical toxicology |
| Volume: | 165 |
| Start Page: | 113136 |
| Publisher DOI: | 10.1016/j.fct.2022.113136 |
| Abstract: | A high-fat diet increases 12 alpha-hydroxylated (12 alpha OH) bile acid (BA) secretion in rats, and secondary BAs are responsible for the leaky gut. This study aimed to examine the role of primary 12 alpha OH BAs in gut barrier impairment in rats using dietary cholic acid (CA) supplementation (0.5 g/kg diet). The CA diet increased the 12 alpha OH BAs concentrations in the small and large intestine, accompanied by gut barrier impairment. Based on the luminal 12 alpha OH BAs concentrations, ex vivo gut leakiness was determined. Deoxycholic acid increased permeability in the large intestine, whereas taurocholic acid (TCA) increased the ileal permeability, but not jejunal permeability. A Rho kinase inhibitor attenuated TCA-induced ileal permeability. Administration of vancomycin, which abolishes secondary BAs, did not influence the gut leakiness induced by the CA diet. Changes in the gut permeation marker in the tail vein blood suggested the possibility that the CA-induced leakiness occurred in the small intestine. The CA diet enhanced the phosphorylation of myosin light chain 2 and reduced claudins expressions in rat ileal epithelia. Reductions in barrier function-related genes were observed in the ileum, but not in the colon of the CA-fed rats. Overall, the present study demonstrated the significance of TCA in proximal gut leakiness. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/86554 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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