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Usefulness of a 3D-printing air sampler for capturing live airborne bacteria and exploring the environmental factors that can influence bacterial dynamics

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Title: Usefulness of a 3D-printing air sampler for capturing live airborne bacteria and exploring the environmental factors that can influence bacterial dynamics
Authors: Mori, Saaya Browse this author
Ishiguro, Sakura Browse this author
Miyazaki, Satoru Browse this author
Okubo, Torahiko Browse this author →KAKEN DB
Omori, Ryosuke Browse this author →KAKEN DB
Kai, Ayako Browse this author
Sugiyama, Kyohei Browse this author
Kawashiro, Airi Browse this author
Sumi, Masato Browse this author
Thapa, Jeewan Browse this author →KAKEN DB
Nakamura, Shinji Browse this author →KAKEN DB
Katoh, Chietsugu Browse this author →KAKEN DB
Yamaguchi, Hiroyuki Browse this author →KAKEN DB
Keywords: 3D printing
Air sampler
Air bacteria
Environmental factors
Issue Date: 1-Sep-2021
Publisher: Elsevier
Journal Title: Research in Microbiology
Volume: 172
Issue: 6
Start Page: 103864
Publisher DOI: 10.1016/j.resmic.2021.103864
PMID: 34273486
Abstract: We created a handmade 3D-printed air sampler to effectively collect live airborne bacteria, and determined which environmental factors influenced the bacteria. Bacterial colony forming units (CFUs) in the air samples (n = 37) were monitored by recording the environmental changes occurring over time, then determining the presence/absence of correlations among such changes. The bacterial CFUs changed sharply and were significantly correlated with the DNA concentrations, indicating that the captured bacteria made up most of the airborne bacteria. Spearman's rank correlation analysis revealed significant correlations between the bacterial CFU values and some environmental factors (humidity, wind speed, insolation, and 24-h rainfall). Similarly the significant associations of CFU with humidity and wind speed were also found by multiple regression analysis with box-cox transformation. Among our panel of airborne bacteria (952 strains), 70 strains were identified as soil-derived Bacillus via the production of Escherichia coli- and Staphylococcus aureus-growth inhibiting antibiotics and by 16S rDNA typing. Soil-derived protozoa were also isolated from the air samples. We conclude that the airborne bacteria mainly derived from soil can alter in number according to environmental changes. Our sampler, which was created by easy-to-customize 3D printing, is a useful device for understanding the dynamics of live airborne bacteria. (C) 2021 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
Rights: © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山口 博之

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