| Title: | Analysis of the Pancreatic Cancer Microbiome Using Endoscopic Ultrasound-Guided Fine-Needle Aspiration-Derived Samples |
| Authors: | Nakano, Shintaro Browse this author |
| Kawamoto, Yasuyuki Browse this author →KAKEN DB |
| Komatsu, Yoshito Browse this author →KAKEN DB |
| Saito, Rika Browse this author |
| Ito, Ken Browse this author |
| Yamamura, Takahiro Browse this author |
| Harada, Kazuaki Browse this author |
| Yuki, Satoshi Browse this author |
| Kawakubo, Kazumichi Browse this author →KAKEN DB |
| Sugiura, Ryo Browse this author |
| Kato, Shin Browse this author |
| Hirata, Koji Browse this author |
| Hirata, Hajime Browse this author |
| Nakajima, Masahito Browse this author |
| Furukawa, Ryutaro Browse this author |
| Takishin, Yunosuke Browse this author |
| Nagai, Kousuke Browse this author |
| Yokota, Isao Browse this author →KAKEN DB |
| Ota, Keisuke H. Browse this author |
| Nakaoka, Shinji Browse this author |
| Kuwatani, Masaki Browse this author →KAKEN DB |
| Sakamoto, Naoya Browse this author →KAKEN DB |
| Keywords: | endoscopic ultrasound-guided fine-needle aspiration |
| bacteria |
| microbiome |
| pancreas |
| pancreatic cancer |
| Issue Date: | Apr-2022 |
| Publisher: | Lippincott Williams & Wilkins (LWW) |
| Journal Title: | Pancreas |
| Volume: | 51 |
| Issue: | 4 |
| Start Page: | 351 |
| End Page: | 357 |
| Publisher DOI: | 10.1097/MPA.0000000000002028 |
| Abstract: | Objectives Most previous studies have analyzed bacteria in tumors using resected pancreatic cancer (PC) tissues, because it is difficult to obtain tissue samples from unresectable advanced PC. We aimed to determine whether minimal tissue obtained by endoscopic ultrasound-guided fine-needle aspiration is useful for microbiome analysis. Methods Thirty PC and matched duodenal and stomach tissues (N = 90) were prospectively collected from 30 patients who underwent endoscopic ultrasound-guided fine-needle aspiration. Bacterial DNA was extracted, and 16S rRNA sequencing was performed. The primary outcome was the success rate of bacterial detection in tumors. Bacterial diversity and structure were investigated. Results The bacterial detection rates were 80%, 100%, and 97% in PC, gastric, and duodenal samples, respectively. Pancreatic cancer tissues showed a lower alpha-diversity and a significantly different microbial structure than stomach and duodenal tissues. Proteobacteria were more abundant, whereas Firmicutes, Bacteroidetes, and Fusobacteria were less abundant in PC tissues than in stomach and duodenal tissues. Acinetobacter was more abundant in PC tissues than in stomach and duodenal tissues, and Delftia was more frequently detected in resectable PC. Conclusions Endoscopic ultrasound-guided fine-needle aspiration samples were valuable for PC microbiome analysis, revealing that the bacterial composition of PC is different from that of the stomach and duodenum. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/86784 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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