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Analysis of the Pancreatic Cancer Microbiome Using Endoscopic Ultrasound-Guided Fine-Needle Aspiration-Derived Samples

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Title: Analysis of the Pancreatic Cancer Microbiome Using Endoscopic Ultrasound-Guided Fine-Needle Aspiration-Derived Samples
Authors: Nakano, Shintaro Browse this author
Kawamoto, Yasuyuki Browse this author →KAKEN DB
Komatsu, Yoshito Browse this author →KAKEN DB
Saito, Rika Browse this author
Ito, Ken Browse this author
Yamamura, Takahiro Browse this author
Harada, Kazuaki Browse this author
Yuki, Satoshi Browse this author
Kawakubo, Kazumichi Browse this author →KAKEN DB
Sugiura, Ryo Browse this author
Kato, Shin Browse this author
Hirata, Koji Browse this author
Hirata, Hajime Browse this author
Nakajima, Masahito Browse this author
Furukawa, Ryutaro Browse this author
Takishin, Yunosuke Browse this author
Nagai, Kousuke Browse this author
Yokota, Isao Browse this author →KAKEN DB
Ota, Keisuke H. Browse this author
Nakaoka, Shinji Browse this author
Kuwatani, Masaki Browse this author →KAKEN DB
Sakamoto, Naoya Browse this author →KAKEN DB
Keywords: endoscopic ultrasound-guided fine-needle aspiration
pancreatic cancer
Issue Date: Apr-2022
Publisher: Lippincott Williams & Wilkins (LWW)
Journal Title: Pancreas
Volume: 51
Issue: 4
Start Page: 351
End Page: 357
Publisher DOI: 10.1097/MPA.0000000000002028
Abstract: Objectives Most previous studies have analyzed bacteria in tumors using resected pancreatic cancer (PC) tissues, because it is difficult to obtain tissue samples from unresectable advanced PC. We aimed to determine whether minimal tissue obtained by endoscopic ultrasound-guided fine-needle aspiration is useful for microbiome analysis. Methods Thirty PC and matched duodenal and stomach tissues (N = 90) were prospectively collected from 30 patients who underwent endoscopic ultrasound-guided fine-needle aspiration. Bacterial DNA was extracted, and 16S rRNA sequencing was performed. The primary outcome was the success rate of bacterial detection in tumors. Bacterial diversity and structure were investigated. Results The bacterial detection rates were 80%, 100%, and 97% in PC, gastric, and duodenal samples, respectively. Pancreatic cancer tissues showed a lower alpha-diversity and a significantly different microbial structure than stomach and duodenal tissues. Proteobacteria were more abundant, whereas Firmicutes, Bacteroidetes, and Fusobacteria were less abundant in PC tissues than in stomach and duodenal tissues. Acinetobacter was more abundant in PC tissues than in stomach and duodenal tissues, and Delftia was more frequently detected in resectable PC. Conclusions Endoscopic ultrasound-guided fine-needle aspiration samples were valuable for PC microbiome analysis, revealing that the bacterial composition of PC is different from that of the stomach and duodenum.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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