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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Leek Yellow Stripe Virus Can Adjust for Host Adaptation by Trimming the N-Terminal Domain to Allow the P1 Protein to Function as an RNA Silencing Suppressor
| Title: | Leek Yellow Stripe Virus Can Adjust for Host Adaptation by Trimming the N-Terminal Domain to Allow the P1 Protein to Function as an RNA Silencing Suppressor |
| Authors: | Sasaki, Jun Browse this author | | Kawakubo, Shusuke Browse this author | | Kim, Hangil Browse this author | | Kim, Ok-Kyung Browse this author | | Yamashita, Kazuo Browse this author | | Shimura, Hanako Browse this author →KAKEN DB | | Masuta, Chikara Browse this author →KAKEN DB |
| Keywords: | alliums | | leek yellow stripe virus | | P1 protein | | garlic | | RNA silencing suppressor |
| Issue Date: | Aug-2022 |
| Publisher: | Korean Society of Plant Pathology |
| Journal Title: | Plant Pathology Journal |
| Volume: | 38 |
| Issue: | 4 |
| Start Page: | 383 |
| End Page: | 394 |
| Publisher DOI: | 10.5423/PPJ.FT.06.2022.0077 |
| Abstract: | In Japan, the P1 protein (S-type) encoded by leek yel-low stripe virus (LYSV) isolates detected in Honshu and southward is shorter than the P1 (N-type) of LYSV isolates from garlic grown in Hokkaido due to a large deletion in the N-terminal half. In garlic fields in Hok-kaido, two types of LYSV isolate with N-and S-type P1s are sometimes found in mixed infections. In this study, we confirmed that N-and S-type P1 sequences were present in the same plant and that they belong to different evolutionary phylogenetic groups. To investi-gate how LYSV with S-type P1 (LYSV-S) could have invaded LYSV with N-type P1 (LYSV-N)-infected gar-lic, we examined wild Allium spp. plants in Hokkaido and found that LYSV was almost undetectable. On the other hand, in Honshu, LYSV-S was detected at a high frequency in Allium spp. other than garlic, suggesting that the LYSV-S can infect a wider host range of Al-liumspp. compared to LYSV-N. Because P1 proteins of potyviruses have been reported to promote RNA silenc-ing suppressor (RSS) activity of HC-Pro proteins, we analyzed whether the same was true for P1 of LYSV. In onion, contrary to expectation, the P1 protein itself had RSS activity. Moreover, the RSS activity of S-type P1 was considerably stronger than that of N-type P1, sug-gesting that LYSV P1 may be able to enhance its RSS activity when the deletion is in the N-terminal half and that acquiring S-type P1 may have enabled LYSV to expand its host range. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/86817 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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