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Suppression of osteoclastogenesis by lactoferrin

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Title: Suppression of osteoclastogenesis by lactoferrin
Authors: Kato, Hiromichi Browse this author
Yoshimura, Yoshitaka Browse this author →KAKEN DB
Minamikawa, Hajime Browse this author →KAKEN DB
Suzuki, Kuniaki Browse this author →KAKEN DB
Yamazaki, Yutaka Browse this author →KAKEN DB
Keywords: lactoferrin
Issue Date: 15-Sep-2022
Publisher: 北海道歯学会
Journal Title: 北海道歯学雑誌
Volume: 43
Start Page: 49
End Page: 56
Abstract: Recent research has shown that lactoferrin indirectly suppresses osteoclastogenesis by affecting osteoblasts and periodontal ligament fibroblasts. However, the mechanism by which lactoferrin directly affects osteoclastogenesis is yet to be reported. Therefore, this study examined the direct effects of lactoferrin on RANKL-induced osteoclast differentiation of murine osteoclastic RAW 264.7 cells. The number of osteoclasts was determined by counting the number of cells positive for tartrate-resistant acid phosphatase staining. The effect of lactoferrin on the number of osteoclasts was measured, and the effect on the mRNA expression of osteoclast differentiation markers was assayed using real-time PCR. Lactoferrin decreased the number of osteoclasts (_2 nuclei) and large osteoclasts (_8 nuclei) in a dose-dependent manner without affecting the viability of RAW 264.7 cells. Additionally, it only mediated these effects within 48 h of culturing the RAW 264.7 cells with RANKL. Lactoferrin also significantly inhibited RANKL-induced mRNA expressions of osteoclastic differentiation genes, such as NFATc1, RANK, DC-STAMP, and OC-STAMP. Thus, these findings suggest that lactoferrin directly suppresses osteoclastogenesis within 48 h of culturing the RAW 264.7 cells with RANKL. Therefore, lactoferrin may be a novel and innovative therapy for treating bone diseases.
Type: article
Appears in Collections:北海道歯学雑誌 > 第43巻

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