| Title: | Morphological Features of the Testis among Autoimmune Mouse Model and Healthy Strains |
| Authors: | Shouman, Zeinab Browse this author |
| Marei, Hany E. Browse this author |
| Abd-Elmaksoud, Ahmed Browse this author |
| Kassab, Mohamed Browse this author |
| Namba, Takashi Browse this author |
| Masum, Md. Abdul Browse this author |
| Elewa, Yaser Hosny Ali Browse this author →ORCID |
| Ichii, Osamu Browse this author →KAKEN DB |
| Kon, Yasuhiro Browse this author →KAKEN DB |
| Keywords: | autoimmune disease |
| immune cell |
| MRL |
| MpJ-Fas(lpr) mice |
| seminiferous tubules |
| Sertoli cells |
| Issue Date: | 5-Aug-2021 |
| Publisher: | Cambridge University Press |
| Journal Title: | Microscopy and microanalysis |
| Volume: | 27 |
| Issue: | 5 |
| Start Page: | 1209 |
| End Page: | 1217 |
| Publisher DOI: | 10.1017/S1431927621012411 |
| Abstract: | Autoimmune diseases play a critical role in the progression of infertility in both sexes and their severity has been reported to increase with age. However, few reports have discussed their effect on the morphological features of the testis. Therefore, we compared the morphological alterations in the testes of autoimmune model mice (MRL/MpJ-Fas(lpr)) and the control strain (MRL/MpJ) with those of their background strain (C57BL/6N) at 3 and 6 months. Furthermore, we analyzed the changes in spermatocytes, Sertoli cells, immune cells, and Zonula occludens-1 junctional protein by immunohistochemical staining. The MRL/MpJ-Fas(lpr) mice showed a significant increase in the serum Anti-double stranded DNA antibody level, relative spleen weight, and seminiferous luminal area when compared with other studied two strains. In contrast, a significant decrease in the relative testis weight, and numbers of both Sertoli, meiotic spermatocyte was observed in MRL/MpJ-Fas(lpr) and MRL/MpJ mice compared with C57BL/6N mice especially at 6 months. Similarly, Zonula occludens-1 junctional protein positive cells showed a significant decrease in the same strains at 6 months. However, no immune cell infiltration could be observed among the studied three strains. Our findings suggest that the increase in autoimmune severity especially with age could lead to infertility through loss of spermatogenic and Sertoli cells, rather than the disturbance of the blood-testis barrier. |
| Rights: | This article has been published in a revised form in [Microscopy and Microanalysis] [http://doi.org/10.1017/S1431927621012411]. This version is published under a Creative Commons CC-BY-NC-ND. No commercial re-distribution or re-use allowed. Derivative works cannot be distributed. ©[Cambridge University Press]. |
| https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/86864 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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