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Morphological Features of the Testis among Autoimmune Mouse Model and Healthy Strains

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Title: Morphological Features of the Testis among Autoimmune Mouse Model and Healthy Strains
Authors: Shouman, Zeinab Browse this author
Marei, Hany E. Browse this author
Abd-Elmaksoud, Ahmed Browse this author
Kassab, Mohamed Browse this author
Namba, Takashi Browse this author
Masum, Md. Abdul Browse this author
Elewa, Yaser Hosny Ali Browse this author →ORCID
Ichii, Osamu Browse this author →KAKEN DB
Kon, Yasuhiro Browse this author →KAKEN DB
Keywords: autoimmune disease
immune cell
MpJ-Fas(lpr) mice
seminiferous tubules
Sertoli cells
Issue Date: 5-Aug-2021
Publisher: Cambridge University Press
Journal Title: Microscopy and microanalysis
Volume: 27
Issue: 5
Start Page: 1209
End Page: 1217
Publisher DOI: 10.1017/S1431927621012411
Abstract: Autoimmune diseases play a critical role in the progression of infertility in both sexes and their severity has been reported to increase with age. However, few reports have discussed their effect on the morphological features of the testis. Therefore, we compared the morphological alterations in the testes of autoimmune model mice (MRL/MpJ-Fas(lpr)) and the control strain (MRL/MpJ) with those of their background strain (C57BL/6N) at 3 and 6 months. Furthermore, we analyzed the changes in spermatocytes, Sertoli cells, immune cells, and Zonula occludens-1 junctional protein by immunohistochemical staining. The MRL/MpJ-Fas(lpr) mice showed a significant increase in the serum Anti-double stranded DNA antibody level, relative spleen weight, and seminiferous luminal area when compared with other studied two strains. In contrast, a significant decrease in the relative testis weight, and numbers of both Sertoli, meiotic spermatocyte was observed in MRL/MpJ-Fas(lpr) and MRL/MpJ mice compared with C57BL/6N mice especially at 6 months. Similarly, Zonula occludens-1 junctional protein positive cells showed a significant decrease in the same strains at 6 months. However, no immune cell infiltration could be observed among the studied three strains. Our findings suggest that the increase in autoimmune severity especially with age could lead to infertility through loss of spermatogenic and Sertoli cells, rather than the disturbance of the blood-testis barrier.
Rights: This article has been published in a revised form in [Microscopy and Microanalysis] []. This version is published under a Creative Commons CC-BY-NC-ND. No commercial re-distribution or re-use allowed. Derivative works cannot be distributed. ©[Cambridge University Press].
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 昆 泰寛

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