| Title: | In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens |
| Authors: | Fujisawa, Sotaro Browse this author |
| Murata, Shiro Browse this author →KAKEN DB |
| Takehara, Masaki Browse this author |
| Aoyama, Julia Browse this author |
| Morita, Ayu Browse this author |
| Isezaki, Masayoshi Browse this author |
| Win, Shwe Yee Browse this author |
| Ariizumi, Takuma Browse this author |
| Sato, Takumi Browse this author |
| Oishi, Eiji Browse this author |
| Taneno, Akira Browse this author |
| Maekawa, Naoya Browse this author →KAKEN DB |
| Okagawa, Tomohiro Browse this author →KAKEN DB |
| Ichii, Osamu Browse this author →KAKEN DB |
| Konnai, Satoru Browse this author →KAKEN DB |
| Ohashi, Kazuhiko Browse this author →KAKEN DB |
| Keywords: | Poultry red mite |
| Adipocyte plasma membrane-associated protein |
| Vaccine |
| Dg-APMAP |
| Dermanyssus gallinae |
| Issue Date: | 1-Oct-2021 |
| Publisher: | Elsevier |
| Journal Title: | Vaccine |
| Volume: | 39 |
| Issue: | 41 |
| Start Page: | 6057 |
| End Page: | 6066 |
| Publisher DOI: | 10.1016/j.vaccine.2021.08.104 |
| Abstract: | The poultry red mite (Dermanyssus gallinae; PRM) is a blood-sucking ectoparasite of chickens that is a threat to poultry farming worldwide and significantly reduces productivity in the egg-laying industry. Chemical acaricides that are widely used in poultry farms for the prevention of PRMs are frequently ineffective due to the emergence of acaricide-resistant PRMs. Therefore, alternative control methods are needed, and vaccination is a promising strategy for controlling PRMs. A novel adipocyte-plasma membrane-associated protein-like molecule (Dg-APMAP) is highly expressed in blood-fed PRMs according to a previous RNA sequencing analysis. Here, we attempted to identify the full sequence of DgAPMAP, study its expression in different life stages of PRMs, and evaluate its potential as a vaccine antigen. Dg-APMAP mRNA was expressed in the midgut and ovaries, and in all life stages regardless of feeding states. Importantly, in vitro feeding of PRMs with plasma derived from chickens immunized with the recombinant protein of the extracellular region of Dg-APMAP significantly reduced their survival rate in nymphs and adults, which require blood meals. Our data suggest that the host immune responses induced by vaccination with Dg-APMAP could be an effective strategy to reduce the suffering caused by PRMs in the poultry industry. (c) 2021 Elsevier Ltd. All rights reserved. |
| Rights: | © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/86867 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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