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In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens

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Title: In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens
Authors: Fujisawa, Sotaro Browse this author
Murata, Shiro Browse this author →KAKEN DB
Takehara, Masaki Browse this author
Aoyama, Julia Browse this author
Morita, Ayu Browse this author
Isezaki, Masayoshi Browse this author
Win, Shwe Yee Browse this author
Ariizumi, Takuma Browse this author
Sato, Takumi Browse this author
Oishi, Eiji Browse this author
Taneno, Akira Browse this author
Maekawa, Naoya Browse this author →KAKEN DB
Okagawa, Tomohiro Browse this author →KAKEN DB
Ichii, Osamu Browse this author →KAKEN DB
Konnai, Satoru Browse this author →KAKEN DB
Ohashi, Kazuhiko Browse this author →KAKEN DB
Keywords: Poultry red mite
Adipocyte plasma membrane-associated protein
Dermanyssus gallinae
Issue Date: 1-Oct-2021
Publisher: Elsevier
Journal Title: Vaccine
Volume: 39
Issue: 41
Start Page: 6057
End Page: 6066
Publisher DOI: 10.1016/j.vaccine.2021.08.104
Abstract: The poultry red mite (Dermanyssus gallinae; PRM) is a blood-sucking ectoparasite of chickens that is a threat to poultry farming worldwide and significantly reduces productivity in the egg-laying industry. Chemical acaricides that are widely used in poultry farms for the prevention of PRMs are frequently ineffective due to the emergence of acaricide-resistant PRMs. Therefore, alternative control methods are needed, and vaccination is a promising strategy for controlling PRMs. A novel adipocyte-plasma membrane-associated protein-like molecule (Dg-APMAP) is highly expressed in blood-fed PRMs according to a previous RNA sequencing analysis. Here, we attempted to identify the full sequence of DgAPMAP, study its expression in different life stages of PRMs, and evaluate its potential as a vaccine antigen. Dg-APMAP mRNA was expressed in the midgut and ovaries, and in all life stages regardless of feeding states. Importantly, in vitro feeding of PRMs with plasma derived from chickens immunized with the recombinant protein of the extracellular region of Dg-APMAP significantly reduced their survival rate in nymphs and adults, which require blood meals. Our data suggest that the host immune responses induced by vaccination with Dg-APMAP could be an effective strategy to reduce the suffering caused by PRMs in the poultry industry. (c) 2021 Elsevier Ltd. All rights reserved.
Rights: © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 村田 史郎

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