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Evaluation of Plant Ceramide Species-Induced Exosome Release from Neuronal Cells and Exosome Loading Using Deuterium Chemistry
| Title: | Evaluation of Plant Ceramide Species-Induced Exosome Release from Neuronal Cells and Exosome Loading Using Deuterium Chemistry |
| Authors: | Murai, Yuta Browse this author →KAKEN DB | | Honda, Takumi Browse this author | | Yuyama, Kohei Browse this author | | Mikami, Daisuke Browse this author | | Eguchi, Koichi Browse this author | | Ukawa, Yuichi Browse this author | | Usuki, Seigo Browse this author | | Igarashi, Yasuyuki Browse this author | | Monde, Kenji Browse this author |
| Keywords: | plant ceramide | | exosome | | amyloid-beta | | Alzheimer's disease | | lipidomics | | deuterium |
| Issue Date: | 15-Sep-2022 |
| Publisher: | MDPI |
| Journal Title: | International Journal of Molecular Sciences |
| Volume: | 23 |
| Issue: | 18 |
| Start Page: | 10751 |
| Publisher DOI: | 10.3390/ijms231810751 |
| Abstract: | The extracellular accumulation of aggregated amyloid-beta (A beta) in the brain leads to the early pathology of Alzheimer's disease (AD). The administration of exogenous plant-type ceramides into AD model mice can promote the release of neuronal exosomes, a subtype of extracellular vesicles, that can mediate A beta clearance. In vitro studies showed that the length of fatty acids in mammalian-type ceramides is crucial for promoting neuronal exosome release. Therefore, investigating the structures of plant ceramides is important for evaluating the potential in releasing exosomes to remove A beta. In this study, we assessed plant ceramide species with D-erythro-(4E,8Z)-sphingadienine and D-erythro-(8Z)-phytosphingenine as sphingoid bases that differ from mammalian-type species. Some plant ceramides were more effective than mammalian ceramides at stimulating exosome release. In addition, using deuterium chemistry-based lipidomics, most exogenous plant ceramides were confirmed to be derived from exosomes. These results suggest that the ceramide-dependent upregulation of exosome release may promote the release of exogenous ceramides from cells, and plant ceramides with long-chain fatty acids can effectively release neuronal exosomes and prevent AD pathology. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/87021 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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