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Mitochondrial maturation in the trophectoderm and inner cell mass regions of bovine blastocysts

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Title: Mitochondrial maturation in the trophectoderm and inner cell mass regions of bovine blastocysts
Authors: Hayashi, Yoshihiro Browse this author
Saito, Shun Browse this author
Bai, Hanako Browse this author →KAKEN DB
Takahashi, Masashi Browse this author →KAKEN DB
Kawahara, Manabu Browse this author →KAKEN DB
Keywords: Bovine
Inner cell mass
Issue Date: Nov-2021
Publisher: Elsevier
Journal Title: Theriogenology
Volume: 175
Start Page: 69
End Page: 76
Publisher DOI: 10.1016/j.theriogenology.2021.08.038
Abstract: Cellular differentiation induces various morphological changes, including elongation, in mitochondria. Preimplantation embryos have round-shaped mitochondria, characteristic of undifferentiated cells. However, there is controversy regarding the precise mitochondrial morphology in blastocyst embryos, which are generated from two cell lineages: undifferentiated inner cell mass (ICM) and differentiated trophectoderm (TE). This study attempted to precisely determine mitochondrial morphology in these two blastocyst regions. Transmission electron microscopy analyses were conducted using more than 1000 mitochondria from blastocyst embryos. No significant differences were observed in the configuration of mitochondrial cristae and frequencies of hooded mitochondria, which are specific to embryos of livestock animals, between the ICM and TE. To accurately compare mitochondrial roundness between the ICM and TE, oblateness was calculated based on both the major and minor axes. Average oblateness was significantly greater in the TE than in the ICM (P < 0.01). These results indicate tissue-specific mitochondrial maturation with complete elongation in the TE at the blastocyst stage. Since mitochondrial elongation is closely associated with cellular metabolism and differentia-tion, the present study provides new insights for better understanding of early embryonic develop-ment in cattle. (c) 2021 Elsevier Inc. All rights reserved.
Rights: ©2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川原 学

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