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Genome-wide DNA methylation profile in feline haematological tumours: A preliminary study

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Title: Genome-wide DNA methylation profile in feline haematological tumours: A preliminary study
Authors: Yamazaki, Jumpei Browse this author →KAKEN DB
Jelinek, Jaroslav Browse this author
Yokoyama, Shoko Browse this author
Takiguchi, Mitsuyoshi Browse this author →KAKEN DB
Keywords: DNA methylation
Next generation sequencing
Issue Date: Nov-2021
Publisher: Elsevier
Journal Title: Research in veterinary science
Volume: 140
Start Page: 221
End Page: 228
Publisher DOI: 10.1016/j.rvsc.2021.09.002
Abstract: Although DNA methylation has been analysed in few studies for a limited number of loci in cats with diseases, genome-wide profile of DNA methylation has never been addressed. The hypothesis for this study is that nextgeneration sequencing with sequential digestion of genomic DNA with SmaI and XmaI enzymes could provide highly quantitative information on methylation levels in cats. Using blood from four healthy control cats and two disease cats as well as three feline lymphoma/leukemia cell lines, approximately 74-94 thousand CpG sites across the cat genome could be analysed. CpG sites in CpG island (CGI) were broadly either methylated or unmethylated in normal blood, while CpG sites in non-CpG islands (NCGI) are largely methylated. Lymphoma cell lines showed thousands of CpG sites with gain of methylation at normally unmethylated CGI sites and loss of methylation at normally methylated NCGI sites. Hypermethylated CpG sites located at promoter regions included genes annotated with 'developmental process' and 'anatomical structure morphogenesis' such as HOXD10. This highly quantitative method would be suitable for studies of DNA methylation changes not only in cancer but also in other common diseases in cats.
Rights: © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山崎 淳平

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