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First molecular detection of Hemolivia and Hepatozoon parasites in reptile-associated ticks on Iriomote Island, Japan
| Title: | First molecular detection of Hemolivia and Hepatozoon parasites in reptile-associated ticks on Iriomote Island, Japan |
| Authors: | Qiu, Yongjin Browse this author →KAKEN DB | | Kidera, Noriko Browse this author | | Thu, May June Browse this author | | Hayashi, Masaki Browse this author | | Fujishima, Kanta Browse this author | | Tamura, Hisao Browse this author |
| Keywords: | Hemolivia | | Hepatozoon | | Amblyomma geoemydae | | Amblyomma nitidum | | Reptile-associated ticks | | Japan |
| Issue Date: | 2-Nov-2021 |
| Publisher: | Springer |
| Journal Title: | Parasitology research |
| Volume: | 120 |
| Start Page: | 4067 |
| End Page: | 4072 |
| Publisher DOI: | 10.1007/s00436-021-07345-y |
| Abstract: | Hepatozoon and Hemolivia are members of the haemogregarines and are reported in reptiles and reptile-associated ticks. However, no studies have reported on Hepatozoon and Hemolivia in Japanese reptile-associated ticks. This study aimed to molecularly identify and to characterize Hepatozoon and Hemolivia in Japanese reptile-associated ticks, Amblyomma geoemydae (Cantor, 1847) and Amblyomma nitidum (Hirst & Hirst, 1910). A total of 41 and 75 DNA samples from A. geoemydae and A. nitidum ticks, respectively, were used for screening of Hepatozoon and Hemolivia with polymerase chain reaction targeting 18S rDNA. As a result, Hemolivia and Hepatozoon were detected in two A. geoemydae and one A. nitidum, respectively. The sequences of Hemolivia spp. showed a 99.5% (1,050/1,055 bp) identity with Hemolivia parvula (KR069083), and the Hemolivia spp. were located in the same clade as H. parvula in the phylogenetic tree. The sequences of Hepatozoon sp. showed a 98.4% (1,521/1,545 bp) identity with Hepatozoon colubri (MN723844), and the Hepatozoon sp. was distinct from validated Hepatozoon species in the tree. Our findings highlight the first molecular record of Hemolivia in Japan and present the first detection of Hepatozoon in A. nitidum. Further investigations on these tick-borne protozoa are required to understand their life cycle and pathogenicity. |
| Rights: | This is a post-peer-review, pre-copyedit version of an article published in Parasitology research. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00436-021-07345-y. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/87062 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 邱 永晋
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