| Title: | Air-liquid interphase culture confers SARS-CoV-2 susceptibility to A549 alveolar epithelial cells |
| Authors: | Sasaki, Michihito Browse this author →KAKEN DB |
| Kishimoto, Mai Browse this author |
| Itakura, Yukari Browse this author |
| Tabata, Koshiro Browse this author |
| Intaruck, Kittiya Browse this author |
| Uemura, Kentaro Browse this author |
| Toba, Shinsuke Browse this author |
| Sanaki, Takao Browse this author |
| Sato, Akihiko Browse this author |
| Hall, William W. Browse this author |
| Orba, Yasuko Browse this author →KAKEN DB |
| Sawa, Hirofumi Browse this author →KAKEN DB |
| Keywords: | SARS-CoV-2 |
| A549 cells |
| Air-liquid interface |
| ACE2 |
| TMPRSS2 |
| Viral entry |
| Issue Date: | Nov-2021 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and biophysical research communications |
| Volume: | 577 |
| Start Page: | 146 |
| End Page: | 151 |
| Publisher DOI: | 10.1016/j.bbrc.2021.09.015 |
| Abstract: | The human lung cell A549 is susceptible to infection with a number of respiratory viruses. However, A549 cells are resistant to Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) infection in conventional submerged culture, and this would appear to be due to low expression levels of the SARSCoV-2 entry receptor: angiotensin-converting enzyme-2 (ACE2). Here, we examined SARS-CoV-2 susceptibility to A549 cells after adaptation to air-liquid interface (ALI) culture. A549 cells in ALI culture yielded a layer of mucus on their apical surface, exhibited decreased expression levels of the proliferation marker KI-67 and intriguingly became susceptible to SARS-CoV-2 infection. We found that A549 cells increased the endogenous expression levels of ACE2 and TMPRSS2 following adaptation to ALI culture conditions. Camostat, a TMPRSS2 inhibitor, reduced SARS-CoV-2 infection in ALI-cultured A549 cells. These findings indicate that ALI culture switches the phenotype of A549 cells from resistance to susceptibility to SARS-CoV-2 infection through upregulation of ACE2 and TMPRSS2. (c) 2021 Elsevier Inc. All rights reserved. |
| Rights: | ©2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/87072 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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