|
|
Hokkaido University Collection of Scholarly and Academic Papers >
Theses >
博士 （農学） >
Understanding the dynamics of soil microbial communities and gas emissions under different soil amendments
|
Files in This Item:
|
|
|
Related Items in HUSCAP:
|
| Title: | Understanding the dynamics of soil microbial communities and gas emissions under different soil amendments |
| Other Titles: | 異なる土壌改良資材施用が土壌微生物叢ダイナミクスとガス排出量に及ぼす影響評価 |
| Authors: | Oraegbunam, Chidozie Johnson Browse this author |
| Issue Date: | 26-Sep-2022 |
| Publisher: | Hokkaido University |
| Abstract: | Soil microbes play important roles in regulating the soil health. The addition of organic materials to the soils can improve the activities of the microbes. Specifically, soil microbes utilize carbon (C) from the applied organic materials to increase their abundance and activities. Contrastingly, soil microbes decompose the added materials and emit the C to the atmosphere as carbon dioxide (CO2). Studies have shown that charred organic materials like biochar can store C in the soil and improve the microbial activities. However, research to verify the impact of biochar on microbial community under different biochar applications is needed. Also, compared to biochar, manure can support microbial activities but the factors regulating the variabilities in manure decomposition are underexplored. First study investigated the effects of different biochar materials on the bacterial community. Second study examined microbial community using network analysis. Third experiment examined the decomposition of cow dung and gas emissions.A pot experiment was conducted to investigate the effects of different materials (chicken manure CM, rice straw RS, and rice husk RH) used to produce biochar on soil microbiome. The biochar was applied as single (CM, RS, and RH), combined form CM+RS, or CM+RH as mixed or surface under a dent corn. In results, surface applications increased the microbial diversity in the soil. This increase was attributed to the increased numbers of OTU such as Actinobacteria and Proteobacteria at the phylum level. Also, RS treatments impacted the microbial richness, and evenness under surface application. Thus, the effect on microbial diversities found in this study depends on the feedstock biochar, therefore biochar materials and application methods should be considered when interpreting its impact on the microbial community.The second study was aimed at investigating the microbial community interactions among different biochar materials using network analysis. Statistical analysis investigating the co-occurrence of microbial taxa were evaluated. The analysis was performed in R software and the network visualizations and correlation statistics were carried out in Gephi software. The results showed dominate phyla to be Proteobacteria, Actinobacteria, and Chloroflexi within the biochar materials. Further, rice husk biochar increased Euryarchaeota, while chicken manure biochar increased Planctomycetes in the soil. Therefore, variabilities of biochar feedstocks should be considered when choosing biochar type for soil amendment because different biochar materials have different impact on the microbial community structure.
The third experiment was carried out to investigate the soil and dung properties influencing the decomposition of cow dung and gas emissions. An incubation study was set-up with the dung and soil sampled from 15 different farms within Hokkaido, Japan. Gas emissions was also measured. During the incubation, samples were taken at three different timings (before, middle, and final incubation) for microbial analysis. Results showed that Firmicutes and Bacteroidetes were significantly decreased while Proteobacteria and Actinobacteria increased during dung decomposition. Also, in each location, there are differences in CO2 emissions pattern which were categorized as high and low CO2 emissions. Following this trend, higher numbers of OTUs were found in low CO2 (3750) compared to the high CO2 (3438). Further insight revealed that soil properties strongly influenced the emissions pattern based on the positive Pearson correlation coefficient between high CO2 emissions and soil properties such as soil C, nitrogen, and CEC. These results indicate that soil properties were the strong determinant of dung decomposition and gas emissions. |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第15142号 |
| Degree Level: | 博士 |
| Degree Discipline: | 農学 |
| Examination Committee Members: | (主査) 准教授 渡部 敏裕, 教授 石黒 宗秀, 教授 当真 要, 准教授 内田 義崇 （国際食資源学院） |
| Degree Affiliation: | 農学院（農学専攻） |
| Type: | theses (doctoral) |
| URI: | http://hdl.handle.net/2115/87144 |
| Appears in Collections: | 課程博士 (Doctorate by way of Advanced Course) > 農学院(Graduate School of Agriculture)
学位論文 (Theses) > 博士 （農学）
|
|
