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Antibacterial tooth surface created by laser-assisted pseudo-biomineralization in a supersaturated solution

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Title: Antibacterial tooth surface created by laser-assisted pseudo-biomineralization in a supersaturated solution
Authors: Oyane, Ayako Browse this author →KAKEN DB
Sakamaki, Ikuko Browse this author
Koga, Kenji Browse this author
Nakamura, Maki Browse this author
Shitomi, Kanako Browse this author →KAKEN DB
Miyaji, Hirofumi Browse this author →KAKEN DB
Keywords: Hydroxyapatite
Calcium phosphate (CaP)
Biomimetic process
Issue Date: Nov-2020
Publisher: Elsevier
Journal Title: Materials science and engineering C : materials for biological applications
Volume: 116
Start Page: 111170
Publisher DOI: 10.1016/j.msec.2020.111170
PMID: 32806265
Abstract: A technique for implementing biocompatible and antibacterial functions to a targeted region on tooth surfaces has potential in dental treatments. We have recently demonstrated pseudo-biomineralization, i.e., the growth of an apatite layer on a human dentin substrate by a laser-assisted biomimetic (LAB) process, based on pulsed laser irradiation in a supersaturated CaP solution. In this study, pseudo-biomineralization was induced in the presence of fluoride ions using the LAB process in order to fabricate an antibacterial fluoride-incorporated apatite (FAp) layer on the dentin surface. After processing for 30 min, a micron-thick FAp layer was formed heterogeneously at the laser-irradiated solid-liquid interface via pseudo-biomineralization. A time-course study revealed that the LAB process first eliminated the pre-existing organic layer, while allowing fluoride incorporation into the dentin surface within 1 min. Within 5 min, FAp nanocrystals precipitated on the dentin surface. Within 30 min, these nanocrystals acquired a pillar-like structure that was weakly oriented in the direction normal to the substrate surface to form a dense micron-thick layer. This layer was integrated seamlessly with the underlying dentin without any apparent gaps. The FAp layer exhibited antibacterial activity against a major oral bacterium, Streptococcus mutans. The proposed LAB process is expected to be a useful new tool for tooth surface functionalization via facile and area-specific pseudo-biomineralization.
Rights: © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宮治 裕史

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