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Hokkaido University Collection of Scholarly and Academic Papers >
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Antibacterial tooth surface created by laser-assisted pseudo-biomineralization in a supersaturated solution
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| Title: | Antibacterial tooth surface created by laser-assisted pseudo-biomineralization in a supersaturated solution |
| Authors: | Oyane, Ayako Browse this author →KAKEN DB | | Sakamaki, Ikuko Browse this author | | Koga, Kenji Browse this author | | Nakamura, Maki Browse this author | | Shitomi, Kanako Browse this author →KAKEN DB | | Miyaji, Hirofumi Browse this author →KAKEN DB |
| Keywords: | Hydroxyapatite | | Fluoride | | Calcium phosphate (CaP) | | Dentin | | Laser | | Biomimetic process | | Coating |
| Issue Date: | Nov-2020 |
| Publisher: | Elsevier |
| Journal Title: | Materials science and engineering C : materials for biological applications |
| Volume: | 116 |
| Start Page: | 111170 |
| Publisher DOI: | 10.1016/j.msec.2020.111170 |
| PMID: | 32806265 |
| Abstract: | A technique for implementing biocompatible and antibacterial functions to a targeted region on tooth surfaces has potential in dental treatments. We have recently demonstrated pseudo-biomineralization, i.e., the growth of an apatite layer on a human dentin substrate by a laser-assisted biomimetic (LAB) process, based on pulsed laser irradiation in a supersaturated CaP solution. In this study, pseudo-biomineralization was induced in the presence of fluoride ions using the LAB process in order to fabricate an antibacterial fluoride-incorporated apatite (FAp) layer on the dentin surface. After processing for 30 min, a micron-thick FAp layer was formed heterogeneously at the laser-irradiated solid-liquid interface via pseudo-biomineralization. A time-course study revealed that the LAB process first eliminated the pre-existing organic layer, while allowing fluoride incorporation into the dentin surface within 1 min. Within 5 min, FAp nanocrystals precipitated on the dentin surface. Within 30 min, these nanocrystals acquired a pillar-like structure that was weakly oriented in the direction normal to the substrate surface to form a dense micron-thick layer. This layer was integrated seamlessly with the underlying dentin without any apparent gaps. The FAp layer exhibited antibacterial activity against a major oral bacterium, Streptococcus mutans. The proposed LAB process is expected to be a useful new tool for tooth surface functionalization via facile and area-specific pseudo-biomineralization. |
| Rights: | © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/87186 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 宮治 裕史
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