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Hokkaido University Collection of Scholarly and Academic Papers >
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Periodontal tissue engineering using an apatite/collagen scaffold obtained by a plasma‐ and precursor‐assisted biomimetic process
| Title: | Periodontal tissue engineering using an apatite/collagen scaffold obtained by a plasma‐ and precursor‐assisted biomimetic process |
| Authors: | Kanemoto, Yukimi Browse this author | | Miyaji, Hirofumi Browse this author →KAKEN DB | | Nishida, Erika Browse this author →KAKEN DB | | Miyata, Saori Browse this author | | Mayumi, Kayoko Browse this author | | Yoshino, Yuto Browse this author | | Kato, Akihito Browse this author →KAKEN DB | | Sugaya, Tsutomu Browse this author →KAKEN DB | | Akasaka, Tsukasa Browse this author →KAKEN DB | | Nathanael, Arputharaj Joseph Browse this author | | Santhakumar, Syama Browse this author | | Oyane, Ayako Browse this author →KAKEN DB |
| Keywords: | bone augmentation | | cyto-compatibility | | dog class II furcation defect model | | immunohistochemistry | | low-crystalline apatite | | osteogenic differentiation | | periodontal healing |
| Issue Date: | 16-Nov-2021 |
| Journal Title: | Journal of Periodontal Research |
| Volume: | 57 |
| Issue: | 1 |
| Start Page: | 205 |
| End Page: | 218 |
| Publisher DOI: | 10.1111/jre.12954 |
| PMID: | 34786723 |
| Abstract: | Background and objectives: In the treatment of severe periodontal destruction, there is a strong demand for advanced scaffolds that can regenerate periodontal tissues with adequate quality and quantity. Recently, we developed a plasma- and precursor-assisted biomimetic process by which a porous collagen scaffold (CS) could be coated with low-crystalline apatite. The apatite-coated collagen scaffold (Ap-CS) promotes cellular ingrowth within the scaffold compared to CS in rat subcutaneous tissue. In the present study, the osteogenic activity of Ap-CS was characterized by cell culture and rat skull augmentation tests. In addition, the periodontal tissue reconstruction with Ap-CS in a beagle dog was compared to that with CS. Methods: The plasma- and precursor-assisted biomimetic process was applied to CS to obtain Ap-CS with a low-crystalline apatite coating. The effects of apatite coating on the scaffold characteristics (i.e., surface morphology, water absorption, Ca release, protein adsorption, and enzymatic degradation resistance) were assessed. Cyto-compatibility and the osteogenic properties of Ap-CS and CS were assessed in vitro using preosteoblastic MC3T3-E1 cells. In addition, we performed in vivo studies to evaluate bone augmentation and periodontal tissue reconstruction with Ap-CS and CS in a rat skull and canine furcation lesion, respectively. Results: As previously reported, the plasma- and precursor-assisted biomimetic process generated a low-crystalline apatite layer with a nanoporous structure that uniformly covered the Ap-CS surface. Ap-CS showed significantly higher water absorption, Ca release, lysozyme adsorption, and collagenase resistance than CS. Cell culture experiments revealed that Ap-CS was superior to CS in promoting the osteoblastic differentiation of MC3T3-E1 cells while suppressing their proliferation. Additionally, Ap-CS significantly promoted (compared to CS) the augmentation of the rat skull bone and showed the potential to regenerate alveolar bone in a dog furcation defect. Conclusion: Ap-CS fabricated by the plasma- and precursor-assisted biomimetic process provided superior promotion of osteogenic differentiation and bone neoformation compared to CS. |
| Rights: | This is the peer reviewed version of the following article: Kanemoto, Y., Miyaji, H., Nishida, E., Miyata, S., Mayumi, K., Yoshino, Y., Kato, A., Sugaya, T., Akasaka, T., Nathanael, A. J., Santhakumar, S., & Oyane, A. (2022). Periodontal tissue engineering using an apatite/collagen scaffold obtained by a plasma- and precursor-assisted biomimetic process. Journal of periodontal research, 57(1), 205–218., which has been published in final form at 10.1111/jre.12954. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/87261 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 宮治 裕史
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