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In situ precipitation of amorphous calcium phosphate nanoparticles within 3D porous collagen sponges for bone tissue engineering

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Title: In situ precipitation of amorphous calcium phosphate nanoparticles within 3D porous collagen sponges for bone tissue engineering
Authors: Santhakumar, Syama Browse this author
Oyane, Ayako Browse this author
Nakamura, Maki Browse this author
Koga, Kenji Browse this author
Miyata, Saori Browse this author
Muratsubaki, Ko Browse this author
Miyaji, Hirofumi Browse this author →KAKEN DB
Keywords: Amorphous calcium phosphate (ACP)
Bone tissue engineering
Basic fibroblast growth factor (bFGF)
Issue Date: Nov-2020
Publisher: Elsevier
Journal Title: Materials Science and Engineering: C
Volume: 116
Start Page: 111194
Publisher DOI: 10.1016/j.msec.2020.111194
PMID: 32806285
Abstract: Amorphous calcium phosphate (ACP) plays an important role in biomineralization within the three-dimensional (3D) collagen network in human hard tissues, and exhibits osteoconductivity. Porous collagen sponges coated with ACP nanoparticles could be considered as potential scaffolds for use in bone tissue engineering. In this study, such composite materials were fabricated via homogeneous ACP precipitation using a supersaturated calcium phosphate (CaP) solution. Homogeneous ACP precipitation was induced in situ within the sponges by a temperature-controlled coating process composed of two steps. In the first step, the CaP solution was cooled to 4 °C to suppress precipitation until the solution penetrated fully into the sponge's internal pores. In the second step, the CaP solution was warmed up to 25 °C with continuous shaking to induce ACP precipitation within the sponges. The resulting sponges were therefore coated with ACP nanoparticles on their inner and outer surfaces. A simulated body fluid (SBF) test indicated osteoconductivity of the collagen sponges coated with ACP nanoparticles. Further, ACP-coated collagen sponges immobilizing basic fibroblast growth factor (bFGF) were fabricated using the CaP solution supplemented with bFGF. The fabricated sponges allowed the sustained release of bFGF in a culture medium and enhanced proliferation of osteoblastic MC3T3-E1 cells. Such ACP-coated collagen sponges have the potential to be used as scaffolds in bone tissue engineering if pursued for further in vitro and in vivo studies.
Rights: © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宮治 裕史

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