Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
BOA/DHB/Na : An Efficient UV-MALDI Matrix for High-Sensitivity and Auto-Tagging Glycomics
Title: | BOA/DHB/Na : An Efficient UV-MALDI Matrix for High-Sensitivity and Auto-Tagging Glycomics |
Authors: | Barada, Erina Browse this author | Hinou, Hiroshi Browse this author →KAKEN DB |
Keywords: | glycomics | MALDI | glycan | oligosaccharide | solid ionic matrix |
Issue Date: | 19-Oct-2022 |
Publisher: | MDPI |
Journal Title: | International Journal of Molecular Sciences |
Volume: | 23 |
Issue: | 20 |
Start Page: | 12510 |
Publisher DOI: | 10.3390/ijms232012510 |
Abstract: | Matrix selection is a critical factor for success in glycomics studies using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). In this study, we evaluated and optimized a new solid ionic matrix-O-benzylhydroxylamine (BOA)/2,5-dihydroxybenzoic acid (DHB)/Na-containing BOA and a small amount of sodium as the counter salt of DHB. The concentration of a mixture of BOA/DHB/Na and glycans on a MALDI target plate led to O-benzyloxy tagging of the reducing ends of the glycans. The BOA/DHB/Na matrix showed excellent aggregation performance and the ability to form a homogeneous solid salt on the MALDI target plate with a water-repellent surface. In addition, the BOA/DHB/Na matrix showed a simple peak pattern with suppressed in-source and post-source decay of the reducing ends of the glycans, as well as improved ionization efficiency of glycans. Utilizing the characteristics of the BOA/DHB/Na matrix, O-glycan analysis of porcine stomach mucin showed excellent detection sensitivity and reproducibility of the peak patterns. This BOA/DHB/Na matrix can accelerate glycomics studies using MALDI-MS and, in combination with other organic salt-type matrices that we have developed, constitutes a valuable tool for glycomics studies. |
Type: | article |
URI: | http://hdl.handle.net/2115/87272 |
Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
|